Immobilization of lysozyme proteins on a hierarchical zeolitic imidazolate framework (ZIF-8)
Immobilization of lysozyme proteins on a hierarchical zeolitic imidazolate framework (ZIF-8)
复制标题
将溶菌酶蛋白固定在分级沸石咪唑酯框架上 (ZIF-8)
DOI：
10.1039/c6dt04582k
复制
发表时间：
2017-02-21
影响因子：
4
通讯作者：
Zhang, Xia
中科院分区：
文献类型：
--
作者：
Liu, Gen;Xu, Yan;Zhang, Xia
以十六烷基三甲基溴化铵为结构控制剂，L-组氨酸为共模板剂，合成了一种多级结构的咪唑骨架分子筛（micro/meso-ZIF-8）。与常规微孔ZIF-8（micro-ZIF-8）相比，微/中孔ZIF- 8的分级多孔结构包含微孔和约35.6nm的最大中孔。所制备的分级微/介孔ZIF-8具有较大的比表面积和对溶菌酶（LZM）、牛血红蛋白（BHb）和牛血清白蛋白（BSA）上级自发吸附活性，且由于分子截断效应，吸附容量随蛋白质尺寸的减小而增大。LZM在micro/meso-ZIF-8上的最大吸附容量高于在类似吸附条件下的大多数报道的结果。吸附动力学和热力学分析表明，吸附机理主要为物理吸附。此外，微/介孔ZIF-8显示出良好的热稳定性对温度和良好的再生能力，在循环吸附实验。本工作为多级结构MOFs在生物分子分离、固定化和富集方面的应用开辟了广阔的前景。
A hierarchical zeolitic imidazolate framework-8 (micro/meso-ZIF-8) was fabricated by using cetyltrimethylammonium bromide as a structure-controlling agent and L-histidine as co-templates. Compared to the conventional microporous ZIF-8 (micro-ZIF-8), the hierarchical porous structure of micro/mesoZIF- 8 contains micropores and maximum mesopores of around 35.6 nm. The as-prepared hierarchical micro/meso-ZIF-8 featured a large surface area and superior spontaneous adsorption activity than micro-ZIF-8 towards lysozyme (LZM), bovine hemoglobin (BHb) and bovine serum albumin (BSA), and the adsorption capacity increased with the decreasing of the protein size due to the molecule cutoff effects. The maximum adsorption capacity of LZM on micro/meso-ZIF-8 was higher than most of the reported results under similar adsorption conditions. The analyses of adsorption kinetics and thermodynamics implied that the adsorption mechanism mainly involved physical adsorption. Moreover, the micro/ meso-ZIF-8 showed good thermal stability against temperature and excellent regeneration ability in the recycling adsorption experiments. This work proposed herein opens a broad application prospect of hierarchical MOFs in biological molecule separation, immobilization and enrichment.