Distribution of glutamate receptor subunit proteins GluR2(4), GluR5/6/7, and NMDAR1 in the canine and primate cerebral cortex: a comparative immunohistochemical analysis.
Distribution of glutamate receptor subunit proteins GluR2(4), GluR5/6/7, and NMDAR1 in the canine and primate cerebral cortex: a comparative immunohistochemical analysis.
复制标题
谷氨酸受体亚基蛋白 GluR2(4)、GluR5/6/7 和 NMDAR1 在犬科动物和灵长类动物大脑皮层中的分布：比较免疫组织化学分析。
DOI：
10.1016/0006-8993(96)00218-1
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发表时间：
1996
期刊：
影响因子：
2.9
通讯作者：
Morrison,JH
中科院分区：
文献类型：
--
作者：
Hof,PR;Vissavajjhala,P;Rosenthal,RE;Fiskum,G;Morrison,JH
分析了AMPA、红藻氨酸和NMDA谷氨酸受体亚单位蛋白G1 uR 2（4）、G1 uR 5/6/7和NMDAR 1在狗海马和新皮质中的分布，并与猕猴和人类进行了比较。在狗海马中，这些谷氨酸受体类表现出相当的分布，在CA 1-CA 3领域的标记的神经元的密度和在齿状回中的neuroglutamine染色模式的差异不大。特别地，G1 uR 5/6/7亚基蛋白的特征在于在CA 1-CA 3区域中的更受限的细胞分布。在狗的新皮层中，与G1 uR 5/6/7或NMDAR 1亚基相比，G1 uR 2（4）亚基在III层和V层的神经元中发现的数量更多，而G1 uR 5/6/7或NMDAR 1亚基主要存在于中到大的V层锥体神经元中。层II和VI始终密集标记的所有三种受体类别，特别是在G1 uR 5/6/7和NMDARI亚基的情况下。迄今为止使用的所有三种抗体都显示出狗大脑皮层中的核周体和树突区段的强烈标记。在某些情况下，顶树突可以穿过几个层，并在所有的新皮层层中形成染色良好的神经丛。这些模式与在猴和人的海马体和新皮质中观察到的模式非常相似，尽管在灵长类动物的皮质神经元群体中比在狗中观察到更多的G1 uR 2（4）和NMDARI免疫反应性。谷氨酸是大脑中主要的兴奋性神经递质，并参与癫痫和脑缺血等病理条件下发生的兴奋性毒性机制。狗已被证明是几种神经系统疾病的可靠大型动物模型，特别是用于心脏骤停的脑反应的研究。在本研究中观察到的狗和灵长类动物的染色模式的整体相似性表明，狗模型可能是非常有价值的表征潜在的细胞和突触的变化，在特定的谷氨酸受体亚基的分布和表达，在其他的生物化学和形态学效应的背景下，全脑缺血和心脏骤停后再灌注。
The distribution of the AMPA, kainate and NMDA glutamate receptor subunit proteins G1uR2(4), G1uR5/6/7 and NMDAR1, respectively, were analyzed in the dog hippocampus and neocortex and compared to macaque monkeys and humans. In the dog hippocampus, these glutamate receptor classes exhibited a comparable distribution with few differences in densities of labeled of neurons in the CA1-CA3 fields and in neuropil staining patterns in the dentate gyrus. In particular, the G1uR5/6/7 subunit proteins were characterized by a more restricted cellular distribution in the CA1-CA3 fields. In the dog neocortex, the G1uR2(4) subunit was found in a higher number of neurons in layers III and V compared to the G1uR5/6/7 or NMDAR1 subunits, which were found predominantly in a population of medium-to-large layer V pyramidal neurons. Layers II and VI were consistently densely labeled with all three receptor classes, especially in the case of the G1uR5/6/7 and NMDARI subunits. All three antibodies used thus far showed an intense labeling of the perikaryon and dendritic segments in the dog cerebral cortex. Apical dendrites could be followed through several layers in some cases, and formed well-stained plexuses in all of the neocortical layers. These patterns were very similar to those observed in the hippocampus and neocortex of both monkey and human, although G1uR2(4), and NMDARI immunoreactivity was visualized in more heterogeneous populations of cortical neurons in the primates than in dogs. Glutamate is the principal excitatory neurotransmitter in the brain and is involved in the excitotoxic mechanisms occurring in pathologic conditions such as epilepsy and cerebral ischemia. The dog has been shown to represent a reliable large animal model for several neurologic disorders and is used particularly in investigations of the cerebral repercussions of cardiac arrest. The overall similarity of the staining patterns in dogs and primates observed in the present study suggest that the dog model may be highly valuable for the characterization of potential cellular and synaptic shifts in the distribution and expression of specific glutamate receptor subunits, in the context of other biochemical and morphologic effects of global brain ischemia and reperfusion following cardiac arrest.