THE SPS4-GENE OF SACCHAROMYCES-CEREVISIAE ENCODES A MAJOR SPORULATION-SPECIFIC MESSENGER-RNA
THE SPS4-GENE OF SACCHAROMYCES-CEREVISIAE ENCODES A MAJOR SPORULATION-SPECIFIC MESSENGER-RNA
复制标题
DOI：
10.1128/mcb.6.12.4478
复制
发表时间：
1986-12-01
影响因子：
5.3
通讯作者：
SEGALL, J
中科院分区：
文献类型：
--
作者：
GARBER, AT;SEGALL, J
The SPS4 gene of Saccharomyces cerevisiae, a sporulation-specific gene identified previously in a differential hybridization screen of a genomic yeast DNA library, has been characterized further. The protein encoded by this gene was inferred from its nucleotide sequence to be 38,600 daltons with an isoelectric pH of 8.2. Consistent with this, two-dimensional polyacrylamide gel electrophoresis of the in vitro translation products of RNA purified by hybridization with the cloned SPS4 DNA indicated that the SPS4 gene product is a 39-kilodalton, basic protein. This protein was found to be identical in size and charge to a major, sporulation-specific protein identified in a two-dimensional polyacrylamide gel electrophoretic comparison of the in vitro translation products of total RNA from sporulating MATa/MAT.alpha. cells and asporogenous MAT.alpha./MAT.alpha. cells. A MATa/MAT.alpha. strain homozygous for a partial deletion of the SPS4 gene appeared, however, to be unaffected in its ability to form viable ascospores.
