Analysis of PBP5 of Early US Isolates of Enterococcus faecium: Sequence Variation Alone Does Not Explain Increasing Ampicillin Resistance over Time
Analysis of PBP5 of Early US Isolates of Enterococcus faecium: Sequence Variation Alone Does Not Explain Increasing Ampicillin Resistance over Time
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DOI：
10.1128/aac.00099-11
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发表时间：
2011-07-01
影响因子：
4.9
通讯作者：
Murray, Barbara E.
中科院分区：
文献类型：
--
作者：
Galloway-Pena, Jessica R.;Rice, Louis B.;Murray, Barbara E.
最近的研究表明，在过去的30年里，美国粪肠球菌分离株对氨苄西林的耐药性稳步上升。对41株分离株预测的PBP5蛋白分析显示，9株MIC值为4mUg/ml的分离株具有一致的PBP5模式，两者之间的差异接近5%；但在后一组中，没有与氨苄西林MICS特异性增加相关的一致的氨基酸变化。对另外三个编码细胞壁/表面蛋白的基因的分析也表明，每个基因有两个不同的进化群体，但偶尔会有基因混合，这与通过重组进化的物种是一致的。
Recent studies have shown that ampicillin resistance has increased steadily over the past 3 decades within U. S. Enterococcus faecium isolates. Analysis of the predicted PBP5 protein of 41 isolates showed a consensus PBP5 pattern for the 9 isolates with MICs of 4 mu g/ml with similar to 5% difference between these; however, there were no consistent amino acid changes that correlated with specific increases in the MICs of ampicillin within the latter group. Analysis of three other genes encoding cell wall/surface proteins also showed that there are two distinct evolutionary groups for each gene, but with occasional mixing of genes, consistent with a species that evolves by recombination.