Development of genic cleavage markers in association with seed glucosinolate content in canola
Development of genic cleavage markers in association with seed glucosinolate content in canola
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开发与双低油菜籽芥子油苷含量相关的基因切割标记
DOI：
10.1007/s00122-015-2487-z
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发表时间：
2015-06-01
影响因子：
5.4
通讯作者：
Qian, Wei
中科院分区：
文献类型：
--
作者：
Fu, Ying;Lu, Kun;Qian, Wei
关键信息在QTL置信区间内鉴定了参与种子硫代葡萄糖甙代谢的油菜直向同源基因，并开发了功能标记，以促进油菜超低硫代葡萄糖甙育种。摘要进一步降低种子硫代葡萄糖甙含量将对油菜种子质量产生积极影响。本研究在德国和中国的7个环境中，利用超亲分离的低硫代葡萄糖甙（GSL）油菜亲本杂交构建了一个双单倍体群体，定位了43个GSL含量的QTL。通过将这些QTL锚定到B.拉帕湾在甘蓝中，我们鉴定了23个在QTL置信区间内参与GSL代谢的拟南芥直向同源物。利用亲本相应编码区之间的序列多态性，开发了两个QTL连锁基因（苹果酸异丙酯脱氢酶1和腺苷5′-磷酸硫酸还原酶3）的切割扩增多态性位点标记。基因切割标记在DH群体中定位到相应的QTL区间，分别解释种子GSL的3.36- 6.88%和4.55- 8.67%的表型变异。这些标记将有助于油菜中超低种子GSL含量的育种。
Key messageThe orthologues ofArabidopsisinvolved in seed glucosinolates metabolism within QTL confidence intervals were identified, and functional markers were developed to facilitate breeding for ultra-low glucosinolates in canola.AbstractFurther reducing the content of seed glucosinolates will have a positive impact on the seed quality of canola (Brassica napus). In this study 43 quantitative trait loci (QTL) for seed glucosinolate (GSL) content in a low-GSL genetic background were mapped over seven environments in Germany and China in a doubled haploid population from a cross between two low-GSL oilseed rape parents with transgressive segregation. By anchoring these QTL to the reference genomes ofB. rapaandB. oleracea,we identified 23 orthologues of Arabidopsis involved in GSL metabolism within the QTL confidence intervals. Sequence polymorphisms between the corresponding coding regions of the parental lines were used to develop cleaved amplified polymorphic site markers for two QTL-linked genes,ISOPROPYLMALATE DEHYDROGENASE1andADENOSINE 5′-PHOSPHOSULFATE REDUCTASE 3. The genic cleavage markers were mapped in the DH population into the corresponding intervals of QTL explaining 3.36–6.88 and 4.55–8.67 % of the phenotypic variation for seed GSL, respectively. The markers will facilitate breeding for ultra-low seed GSL content in canola.