Laser Scanning In Vivo Confocal Microscopy of Clear Grafts after Penetrating Keratoplasty.
Laser Scanning In Vivo Confocal Microscopy of Clear Grafts after Penetrating Keratoplasty.
复制标题
穿透性角膜移植术后透明移植物的激光体内共焦显微镜检查
DOI：
10.1155/2016/5159746
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发表时间：
2016
影响因子：
--
通讯作者：
Gao H
中科院分区：
文献类型：
--
作者：
Wang D;Song P;Wang S;Sun D;Wang Y;Zhang Y;Gao H
目的。通过体内激光扫描共聚焦显微镜评估穿透性角膜移植术（PK）后中央透明移植物中角膜细胞和树突状细胞的变化。方法。选取30名在山东眼科研究所接受PK且移植物清晰且术后无免疫排斥反应的成年受试者纳入本研究，并以10名健康成人为对照。通过激光扫描共聚焦显微镜评估中央移植物中的角膜细胞和树突细胞，以及上皮细胞、角膜细胞、角膜内皮细胞和角膜神经（特别是上皮下丛神经）。结果。透明移植物中上皮下丛神经的中位密度、基质各层角膜细胞密度和角膜内皮细胞密度均低于对照组。 PK 后，5 名患者 (16.7%) 的鲍曼膜和移植物基质膜中的树突状细胞活跃。结论。在一些透明移植物中可以检测到活化的树突状细胞和朗格汉斯细胞，这表明即使没有临床排斥反应，免疫反应的亚临床应激也参与了PK后透明移植物的慢性损伤。
Purpose. To evaluate the changes of keratocytes and dendritic cells in the central clear graft by laser scanning in vivo confocal microscopy after penetrating keratoplasty (PK). Methods. Thirty adult subjects receiving PK at Shandong Eye Institute and with clear grafts and no sign of immune rejection after surgery were recruited into this study, and 10 healthy adults were controls. The keratocytes and dendritic cells in the central graft were evaluated by laser scanning confocal microscopy, as well as epithelium cells, keratocytes, corneal endothelium cells, and corneal nerves (especially subepithelial plexus nerves). Results. Median density of subepithelial plexus nerves, keratocyte density in each layer of the stroma, and density of corneal endothelium cells were all lower in clear grafts than in controls. The dendritic cells of five (16.7%) patients were active in Bowman's membrane and stromal membrane of the graft after PK. Conclusions. Activated dendritic cells and Langerhans cells could be detected in some of the clear grafts, which indicated that the subclinical stress of immune reaction took part in the chronic injury of the clear graft after PK, even when there was no clinical rejection episode.