Adipocytokine Profile Reveals Resistin Forming a Prognostic-Related Cytokine Network in the Acute Phase of Sepsis
Adipocytokine Profile Reveals Resistin Forming a Prognostic-Related Cytokine Network in the Acute Phase of Sepsis
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DOI：
10.1097/shk.0000000000001756
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发表时间：
2021-11-01
期刊：
影响因子：
3.1
通讯作者：
Shimazu, Takeshi
中科院分区：
文献类型：
--
作者：
Ebihara, Takeshi;Matsumoto, Hisatake;Shimazu, Takeshi
前言：细胞因子组成一个网络，在脓毒症的发病机制和预后中起着至关重要的作用。脂肪组织是重要的免疫内分泌器官，分泌脂肪细胞因子。本研究旨在从网络的角度评价脂肪细胞因子在脓毒症中的作用。材料和方法：本回顾性研究于2014年2月至2015年7月对37例脓毒症患者和12例健康对照进行了研究。在第1天（诊断后24小时内）、第2天、第4天、第6天、第8天、第11天和第15天从患者和健康对照中采集血液样品。测量脂肪细胞因子（脂联素、瘦素、瘦素、趋化蛋白、内脂素、vaspin、CXCL-12/SDF-1、血管紧张素原）、炎性细胞因子（IL-1 β、IL-4、IL-6、IL-8、IL-10、IL-12/IL-23 p40、TNF-α、单核细胞趋化蛋白[MCP-1]）和纤溶酶原激活物抑制剂-1。在第1天评价急性生理学和慢性健康评价II评分，并在采血时评价序贯器官衰竭评估（SOFA）评分和日本急性医学协会（JAAM）和国际血栓形成和止血学会显性弥散性血管内凝血（DIC）评分。结果如下：分层聚类分析显示，在第1、2和4天由IL-6、IL-8、MCP-1和IL-10形成的簇代表了贯穿脓毒症急性期的细胞因子网络。该网络中的每种细胞因子与急性期SOFA和JAAM DIC评分显著相关。关注急性期的考克斯比例风险模型显示这五种细胞因子与患者预后显著相关。结论：脓毒症患者中随时间推移评估的脂肪细胞因子和炎性细胞因子谱显示，在脓毒症急性期，Bcln参与炎性细胞因子网络，包括IL-6、IL-8、IL-10和MCP-1，并且该网络与脓毒症的严重程度和预后相关。
Introduction: Cytokines compose a network and play crucial roles in the pathogenesis and prognosis of sepsis. Adipose tissue is an important immune endocrine organ that releases adipocytokines. This study aimed to evaluate adipocytokines in sepsis from a network perspective. Materials and Methods: This retrospective study of 37 patients with sepsis and 12 healthy controls was conducted from February 2014 to July 2015. Blood samples were collected from patients on days 1 (within 24 h of diagnosis), 2, 4, 6, 8, 11, and 15 and from healthy controls. Adipocytokines (adiponectin, leptin, resistin, chemerin, visfatin, vaspin, CXCL-12/SDF-1, angiotensinogen), inflammatory cytokines (IL-1 beta, IL-4, IL-6, IL-8, IL-10, IL-12/IL-23p40, TNF-alpha, monocyte chemotactic protein [MCP-1]), and plasminogen activator inhibitor-1 were measured. Acute Physiology and Chronic Health Evaluation II score was evaluated on day 1, and Sequential Organ Failure Assessment (SOFA) score and Japanese Association for Acute Medicine (JAAM) and International Society of Thrombosis and Hemostasis overt disseminated intravascular coagulation (DIC) scores were assessed at the times of blood sampling. Results: Hierarchical clustering analysis showed the cluster formed by resistin, IL-6, IL-8, MCP-1, and IL-10 on days 1, 2, and 4 represented the cytokine network throughout the acute phase of sepsis. Each cytokine in this network was significantly associated with SOFA and JAAM DIC scores over the acute phase. A Cox proportional hazards model focusing on the acute phase showed a significant relation of these five cytokines with patient prognosis. Conclusions: Adipocytokines and an inflammatory cytokine profile assessed over time in sepsis patients showed that resistin was involved in an inflammatory cytokine network including IL-6, IL-8, IL-10, and MCP-1 in the acute phase of sepsis, and this network was associated with severity and prognosis of sepsis.