Characterization of heat shock protein 70 transcript from Nilaparvata lugens (Stal): Its response to temperature and insecticide stresses
Characterization of heat shock protein 70 transcript from Nilaparvata lugens (Stal): Its response to temperature and insecticide stresses
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褐飞虱 (Stal) 热休克蛋白 70 转录物的表征：其对温度和杀虫剂胁迫的响应
DOI：
10.1016/j.pestbp.2017.01.011
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发表时间：
2017-10-01
影响因子：
4.7
通讯作者：
Zhou, Qiang
中科院分区：
文献类型：
--
作者：
Lu, Kai;Chen, Xia;Zhou, Qiang
褐飞虱，Nilaparvata lugens，对极端温度和杀虫剂胁迫具有很强的适应性。热休克蛋白（Hsps）是一种高度保守的分子伴侣蛋白，在昆虫对各种环境胁迫的反应中起着关键作用。然而，对热休克蛋白在N. lugens中对应激的反应知之甚少。本研究从该昆虫中分离到诱导型热休克蛋白70 (NIHsp70)，分析了NIHsp70在温度和杀虫剂胁迫下的转录表达模式。NIHsp70全长2805 bp，开放阅读框（ORF）为1896 bp，与其他物种同源性较高。NIHsp70的表达在热休克1小时内没有改变，在热应激恢复后也没有改变。相反，NIHsp70的表达在冷休克后下降。此外，吡虫啉暴露后NIHsp70的表达增加。RNA干扰实验和杀虫剂伤害实验也证实了NIHsp70在抗杀虫剂暴露中起重要作用。这些结果表明，NIHsp70是一个重要的基因，参与了真菌对环境胁迫的抗性或耐受性。有趣的是，在吡虫啉暴露的褐飞虱成虫中，NIHsp90和NIHsc70两个热诱导型热休克蛋白基因的mRNA表达水平发生了微弱的变化，这表明不同的热休克蛋白可能对极端温度和杀虫剂胁迫有不同的反应。(C) 2017爱思唯尔公司版权所有。
The brown planthopper, Nilaparvata lugens, possesses a strong adaptability to extreme temperature and insecticide stresses. Heat shock proteins (Hsps) are highly conserved molecular chaperones and play a pivotal role in response to various environmental stresses in insects. However, little is known about the response of Hsps to stresses in N. lugens. In the present study, an inducible Hsp70 (NIHsp70) was isolated from this insect and transcriptional expression patterns of NIHsp70 under temperature and insecticide stresses were analyzed. The full-length of NIHsp70 was 2805 bp with an open reading frame (ORF) of 1896 bp, showing high homology to its counterparts in other species. Expression of NIHsp70 was not altered by heat shock for 1 h, nor following recovery from thermal stress. Conversely, decreased expression of NIHsp70 was observed in response to cold shock. In addition, the expression of NIHsp70 increased after imidacloprid exposure. RNA interference experiment combined with insecticide injury assay also demonstrated that NIHsp70 was essential for resistance against insecticide exposure. These observations indicated that NIHsp70 was an important gene involved in the resistance or tolerance to environmental stresses in N. lugens. Interestingly, weak changes in mRNA expression levels of two thermal-inducible Hsp genes, NIHsp90 and NIHsc70 were observed in imidacloprid-exposed N. lugens adults, suggesting that different Hsps may respond differential to the extreme temperature and insecticide stresses. (C) 2017 Elsevier Inc. All rights reserved.