Novel Soft Alginate Hydrogel Strongly Supports Neurite Growth and Protects Neurons Against Oxidative Stress
Novel Soft Alginate Hydrogel Strongly Supports Neurite Growth and Protects Neurons Against Oxidative Stress
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DOI：
10.1089/ten.tea.2011.0097
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发表时间：
2012-01-01
影响因子：
4.1
通讯作者：
Ikonomidou, Chrysanthy
中科院分区：
文献类型：
--
作者：
Matyash, Marina;Despang, Florian;Ikonomidou, Chrysanthy
神经组织工程的重点是开发生物材料，可以支持创伤后神经元的再生，以及由退行性疾病引起的损伤。在这项工作中，我们描述了新的软藻酸盐水凝胶，它提供了一个大鼠和人类神经元的粘合剂基质，促进神经突生长。只有软的水凝胶，制备与亚化学计量浓度的Ca2+，Ba2+，和Sr2+阳离子的交联与不>10%的所有潜在可用的凝胶化位点在藻酸盐，促进快速和丰富的神经突生长在原代神经元单层培养物，神经球体，神经元衍生自大鼠和人类神经干细胞。为了支持神经突生长，水凝胶不需要任何细胞外基质成分的修饰，并且由不同来源的高粘度和低粘度藻酸盐制备。此外，在软水凝胶上培养的神经元对过氧化氢诱导的氧化应激损伤具有抵抗性。这些发现适用于大鼠和人类神经元，超越了藻酸盐作为细胞封装惰性材料的作用。这样的软藻酸盐水凝胶可用于制备用于预防和治疗神经变性疾病、用于促进外周和中枢神经系统中的神经元再生以及用于神经组织工程应用的药物组合物。
Neural tissue engineering focuses on development of biomaterials that could support regeneration of neurons after trauma as well as injury caused by degenerative diseases. In this work we describe novel soft alginate hydrogels, which provide an adhesive matrix for rat and human neurons and facilitate neurite outgrowth. Only soft hydrogels, prepared with sub-stoichiometric concentrations of Ca2+, Ba2+, and Sr2+ cations by cross-linking with no >10% of all potentially available gelation sites in alginate, facilitated rapid and abundant neurite outgrowth in primary neuronal monolayer cultures, neural spheroids, and neurons derived from rat and human neural stem cells. To support neurite growth, hydrogels did not require modification by any extracellular matrix components and were prepared from high as well as low viscous alginates of different origin. In addition, neurons cultured on soft hydrogels were resistant to oxidative stress injury induced by hydrogen peroxide. These findings, which apply both to rat and human neurons, go beyond the well-described role of alginates as inert materials for cell encapsulation. Such soft alginate hydrogels may be useful for the preparation of pharmaceutical compositions for prophylaxis and treatment of neurodegenerative disorders, for promoting neuronal regeneration in the peripheral and central nervous system and for neural tissue engineering applications.