Fragment length polymorphisms among independent isolates of Epstein-Barr virus from immunocompromised and normal hosts.
Fragment length polymorphisms among independent isolates of Epstein-Barr virus from immunocompromised and normal hosts.
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来自免疫功能低下和正常宿主的 Epstein-Barr 病毒独立分离株之间的片段长度多态性。
DOI：
10.1093/infdis/157.2.299
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Miller,G
中科院分区：
文献类型：
--
作者：
Katz,BZ;Niederman,JC;Olson,BA;Miller,G
EB病毒（Epstein-Barr virus，EBV）DNA的限制性片段长度多态性被用作分子流行病学工具，用于研究来自相同和不同个体的多个病毒分离株。我们研究了35株EB病毒分离株：19株来自7名免疫功能低下的儿童，16株来自7名患有单核细胞增多症的大学生。在此收集的分离株的片段长度多态性分析允许几个结论。多态性位点最常出现在重复DNA区域。流行病学上无关的患者携带的病毒很容易区分;相比之下，两名婴儿及其母亲携带的病毒相似。来自同一患者不同部位的分离株相似。不同的EBV临床分离株之间的变异与不同的实验室病毒株之间的变异相似。因此，片段长度多态性为研究EBV感染的传播和发病机制提供了有用的标记。
DNA restriction fragment length polymorphisms of Epstein-Barr virus (EBV) DNA were used as a molecular epidemiological tool to study multiple isolates of virus from the same and different individuals. We studied 35 EBV isolates: 19 from seven immunocompromised children and 16 from seven college students with mononucleosis. Analysis of the fragment length polymorphisms in this collection of isolates permitted several conclusions. Sites of polymorphism were most often encountered in regions with repetitive DNA. Epidemiologically unrelated patients harbored viruses that could be readily distinguished; by contrast, two infants and their mothers harbored similar viruses. Isolates from different sites in the same patient were similar. Variations between different clinical isolates of EBV mimic those found between different laboratory strains of the virus. Fragment length polymorphisms thus provide a useful marker for studying transmission and pathogenesis of EBV infections.