The mitochondrial genome of the onychophoran Opisthopatus cinctipes (Peripatopsidae) reflects the ancestral mitochondrial gene arrangement of Panarthropoda and Ecdysozoa
The mitochondrial genome of the onychophoran Opisthopatus cinctipes (Peripatopsidae) reflects the ancestral mitochondrial gene arrangement of Panarthropoda and Ecdysozoa
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DOI：
10.1016/j.ympev.2010.05.011
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发表时间：
2010-10-01
影响因子：
4.1
通讯作者：
Mayer, Georg
中科院分区：
文献类型：
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作者：
Braband, Anke;Cameron, Stephen L.;Mayer, Georg
Onychophora（天鹅绒蠕虫）的祖先基因组组成是未知的，因为迄今为止只研究了Peripatidae的一个物种，这表明了一个高度衍生的基因顺序，具有许多易位基因。由于缺乏来自甲门的信息，很难推断出泛节肢动物门和蜕皮动物门的祖先线粒体基因排列模式。因此，我们分析了全线粒体基因组的onychophoran Opisthopatus cinctipes，Peripatopsidae的代表。我们的数据表明O.带足类动物具有高度保守的基因顺序，与在各种节肢动物中发现的基因顺序相似。通过与不同外类群的结果比较，我们重建了泛节肢动物和蜕皮动物的祖先基因排列。我们的蛋白质编码基因序列的系统发育分析，从60个原口类物种（包括外类群）提供了一些支持的姐妹组关系的Onychophora和节肢动物，这是没有恢复使用一个单一的物种Peripatidae，Epiperipatus biolleyi，在以前的研究。甲，节肢动物和priapulid之间的链特异性的偏见的比较表明，peripatid E。biolleyi比peripatopsid O.带爪的(C)2010年爱思唯尔公司All rights reserved.
The ancestral genome composition in Onychophora (velvet worms) is unknown since only a single species of Peripatidae has been studied thus far, which shows a highly derived gene order with numerous translocated genes. Due to this lack of information from Onychophora, it is difficult to infer the ancestral mitochondrial gene arrangement patterns for Panarthropoda and Ecdysozoa. Hence, we analyzed the complete mitochondrial genome of the onychophoran Opisthopatus cinctipes, a representative of Peripatopsidae. Our data show that O. cinctipes possesses a highly conserved gene order, similar to that found in various arthropods. By comparing our results to those from different outgroups, we reconstruct the ancestral gene arrangement in Panarthropoda and Ecdysozoa. Our phylogenetic analysis of protein-coding gene sequences from 60 protostome species (including outgroups) provides some support for the sister group relationship of Onychophora and Arthropoda, which was not recovered by using a single species of Peripatidae, Epiperipatus biolleyi, in a previous study. A comparison of the strand-specific bias between onychophorans, arthropods, and a priapulid suggests that the peripatid E. biolleyi is less suitable for phylogenetic analyses of Ecdysozoa using mitochondrial genomic data than the peripatopsid O. cinctipes. (C) 2010 Elsevier Inc. All rights reserved.