Integrity of bacterial genomic DNA after autoclaving: possible implications for horizontal gene transfer and clinical waste management
Integrity of bacterial genomic DNA after autoclaving: possible implications for horizontal gene transfer and clinical waste management
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DOI：
10.1016/j.jhin.2012.11.016
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发表时间：
2013-03-01
影响因子：
6.9
通讯作者：
Moore, J. E.
中科院分区：
文献类型：
--
作者：
Yap, J. M.;Goldsmith, C. E.;Moore, J. E.
目前的高压灭菌方法旨在杀死细菌。关于高压灭菌对细菌基因组 DNA 完整性的影响知之甚少。为了检验标准高压灭菌对细菌 DNA 完整性的影响，我们进行了一项实验，采用聚合酶链式反应 (PCR) 作为 DNA 完整性的指标。对于铜绿假单胞菌 NCTC 10662，在高压灭菌时间 t = 10、20 和 30 分钟时观察到可扩增的 PCR 信号；对于诺丁汉沙门氏菌 NCTC 7832，t = 10、20、30 和 40 分钟；对于大肠杆菌 NCTC 9001，t = 10 和 20 分钟。因此，在未来的风险和环境影响评估中，应仔细考虑残留分子假象，其中来自死亡细菌和高等生物体的残留基因组 DNA 可能充当潜在的储存库，从而为活细胞中的水平基因转移场景提供毒力、持久性或抗生素抗性特征的潜在危险基因。 (C) 2012 年医疗保健感染协会。由爱思唯尔有限公司出版。保留所有权利。
Current autoclaving practices are designed to kill bacteria. Little is known about the effect of autoclaving on the integrity of bacterial genomic DNA. An experiment was performed to examine the effect of standard autoclaving on the integrity of bacterial DNA, employing polymerase chain reaction (PCR) as an indicator of DNA integrity. Amplifiable PCR signal was observed at t = 10, 20 and 30 min autoclaving time for Pseudomonas aeruginosa NCTC 10662; at t = 10, 20, 30 and 40 min for Salmonella Nottingham NCTC 7832; and at t = 10 and 20 min for Escherichia coli NCTC 9001. Careful consideration should therefore be given to residual molecular artefacts in future risk and environmental impact assessments, where the legacy of residual genomic DNA from dead bacterial and higher organisms may act as a potential reservoir, thereby feeding horizontal gene transfer scenarios in viable cells with potential hazardous genes of virulence, persistence or antibiotic resistance characteristics. (C) 2012 The Healthcare Infection Society. Published by Elsevier Ltd. All rights reserved.