Surface-enhanced Raman scattering studies of human transcriptional coactivator p300
Surface-enhanced Raman scattering studies of human transcriptional coactivator p300
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DOI：
10.1021/jp063071e
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发表时间：
2006-08-24
影响因子：
3.3
通讯作者：
Narayana, Chandrabhas
中科院分区：
文献类型：
--
作者：
Kumar, G. V. Pavan;Reddy, B. A. Ashok;Narayana, Chandrabhas
本文首次报道了p300蛋白的表面增强拉曼散射（Sers）研究。振动光谱分析已经进行了尝试，以了解在其晶体结构的情况下的p300的结构。在蛋白质光谱中观察到与酰胺I-III相关的强拉曼带。这已经通过对氘代p300进行Sers证实。我们还观察到与α-螺旋、色氨酸、苯丙氨酸、酪氨酸和组氨酸相关的拉曼带。这些谱带为研究药物与蛋白质的相互作用提供了理想的工具。我们已经成功地证明了氯离子对p300的Sers的影响。加入氯离子后，Sers谱中的拉曼强度增加，沿着出现新的模式。我们发展了一种新的方法，即“三明治技术”，它可以用来在干燥条件下进行蛋白质的Sers实验。
We report for the first time the surface-enhanced Raman scattering (SERS) studies on p300, a large multidomain transcriptional coactivator protein. Vibration spectral analysis has been performed in an attempt to understand the structure of the p300 in the absence of its crystal structure. Strong Raman bands associated with amides I-III have been observed in the protein spectra. This has been confirmed by performing SERS on deuterated p300. We also observe Raman bands associated with the alpha-helix, tryptophan, phenylalanine, tyrosine, and histidine. These bands will provide an ideal tool to study the drug-protein interactions in therapeutics using SERS. We have successfully demonstrated the chloride ion effect on the SERS of p300. The Raman intensity increases in the SERS spectra upon addition of chloride ion along with appearance of new modes. We have developed a new method, namely, the "sandwich technique", which could be used to perform SERS experiments on proteins in dry conditions.