Application of differential scanning microcalorimetry to the study of cellular processes: heat production and glucose oxidation of murine macrophages.
Application of differential scanning microcalorimetry to the study of cellular processes: heat production and glucose oxidation of murine macrophages.
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差示扫描微量热法在细胞过程研究中的应用：鼠巨噬细胞的产热和葡萄糖氧化。
DOI：
10.1073/pnas.78.10.5958
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发表时间：
1981
影响因子：
11.1
通讯作者：
Sturtevant,JM
中科院分区：
文献类型：
--
作者：
Loike,JD;Silverstein,SC;Sturtevant,JM
差示扫描量热法为研究活细胞的热演化提供了一种非侵入性的方法。我们使用这项技术测量了硫代乙醇酸盐肉汤诱导的小鼠巨噬细胞的放热，以及NaF、KCN、放线菌亚胺和细胞松弛素B和D对这一参数的影响。根据细胞密度、葡萄糖浓度和各种药物的存在或不存在，在1℃最低温度下扫描的10-37℃区间内，每个细胞的总热量范围为300至2500×10(-12)卡(1Cal=4.184 J)。
Differential scanning microcalorimetry provides a noninvasive method for studying heat evolution in living cells. We used this technique to measure the heat evolved by thioglycollate broth-elicited mouse macrophages, and the effects of NaF, KCN, cycloheximide, and cytochalasins B and D on this parameter. The total heat evolved in the interval 10--37 degrees C scanned at 1 degree C min-1 ranged from 300 to 2500 X 10(-12) cal (1 Cal = 4.184 J) per cell, depending on cell density, glucose concentration, and the presence or absence of various drugs.