LOCALIZATION OF DIFFERENT FORMS OF TROPONIN-I IN SKELETAL AND CARDIAC-MUSCLE CELLS
LOCALIZATION OF DIFFERENT FORMS OF TROPONIN-I IN SKELETAL AND CARDIAC-MUSCLE CELLS
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DOI：
10.1016/0014-4827(78)90149-0
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发表时间：
1978-01-01
影响因子：
3.7
通讯作者：
PERRY, SV
中科院分区：
文献类型：
--
作者：
DHOOT, GK;GELL, PGH;PERRY, SV
从人心脏和兔快骨骼肌和慢骨骼肌中分离的抗肌钙蛋白I的抗体显示出对它们所针对的肌钙蛋白I的多态形式具有特异性，即，它们具有组织特异性。这些抗体与肌钙蛋白I的多态性形式反应，它们是针对肌钙蛋白I产生的，肌钙蛋白I存在于其他物种如恒河猴、仓鼠和大鼠的组织中，即，它们是非特定物种。使用免疫过氧化物酶染色技术，肌钙蛋白I的快速和慢速形式位于不同的细胞中，几乎所有的成年正常肌肉检查。通过比较在pH 9.4和4.2下骨骼肌切片的ATP酶染色，肌钙蛋白I的快速形式明显位于II型纤维中，而慢速形式位于I型纤维中。用肌钙蛋白I的快型和慢型抗体进行免疫过氧化物酶染色提供了一种明确的肌纤维分型新方法。
Antibodies raised against troponin I isolated from human cardiac and rabbit fast and slow skeletal muscles were shown to be specific for the polymorphic forms of troponin I against which they were raised, i.e., they are tissue specific. These antibodies reacted with the polymorphic forms of troponin I, against which they were raised, that are present in tissues of other species such as the rhesus monkey, hamster and rat, i.e., they were species non-specific. Using the immunoperoxidase staining technique, the fast and slow forms of troponin I are located in different cells in virtually all adult normal muscles examined. By comparison of the ATPase staining of skeletal muscle sections at pH 9.4 and 4.2 the fast form of troponin I is apparently located in type II fibers and the slow form in type I fibers. Immunoperoxidase staining with the antibodies to the fast and slow forms of troponin I provides an unambiguous new method of muscle fiber typing.