Hippocampal Granule Neuron Number and Dentate Gyrus Volume in Antidepressant-Treated and Untreated Major Depression
Hippocampal Granule Neuron Number and Dentate Gyrus Volume in Antidepressant-Treated and Untreated Major Depression
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DOI：
10.1038/npp.2013.5
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发表时间：
2013-05-01
影响因子：
7.6
通讯作者：
Mann, J. John
中科院分区：
文献类型：
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作者：
Boldrini, Maura;Santiago, Adrienne N.;Mann, J. John
据报道，重度抑郁症（MDD）的海马体积较小。我们推测这可能与齿状回（DG）中颗粒神经元（GN）较少有关，这种缺陷可能通过抗抑郁药逆转。我们研究了年龄、性别和死后间隔匹配的组：无主要精神病理学（对照组）;未用药的MDD;以及用5-羟色胺再摄取抑制剂（MDD*SSRI）或三环类药物（MDD*TCA）治疗的MDD。将冷冻的右心室固定，切片（50 μ m），用神经元核标记物（NeuN）免疫染色，并用苏木精复染。GN和胶质细胞的数量，DG和颗粒细胞层（GCL）的体积进行了体视学估计。与对照组相比，未用药MDD中前DG中的GN较少（p = 0.013）。MDD发病年龄越小，GN越少（p = 0.021）。未用药的MDD的中期DG GN少于MDD*SSRIs（p = 0.028）和对照组（p = 0.032）。前GCL神经胶质细胞的数量没有组间差异。与对照组相比，未用药MDD组的前/中GCL体积较小（p = 0.008），MDD*SSRIs组的前/中GCL体积较大（p
Smaller hippocampal volume is reported in major depressive disorder (MDD). We hypothesize that it may be related to fewer granule neurons (GN) in the dentate gyrus (DG), a defect possibly reversible with antidepressants. We studied age-, sex-, and postmortem interval-matched groups: no major psychopathology (controls); unmedicated-MDD; and MDD treated with serotonin reuptake inhibitors (MDD*SSRI) or tricyclics (MDD*TCA). Frozen right hippocampi were fixed, sectioned (50 mu m), immunostained with neuronal nuclear marker (NeuN), and counterstained with hematoxylin. GN and glial number, and DG and granule cell layer (GCL) volumes were stereologically estimated. Fewer GNs in the anterior DG were present in unmedicated-MDDs compared with controls (p = 0.013). Younger age of MDD onset correlated with fewer GNs (p = 0.021). Unmedicated-MDDs had fewer mid-DG GNs than MDD*SSRIs (p = 0.028) and controls (p = 0.032). Anterior GCL glial number did not differ between groups. Anterior/mid GCL volume was smaller in unmedicated-MDDs vs controls (p = 0.008) and larger in MDD*SSRIs vs unmedicated-MDDs (p