Intestinal microbiota and immunoglobulin E responses in 5-year-old Estonian children
Intestinal microbiota and immunoglobulin E responses in 5-year-old Estonian children
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DOI：
10.1111/j.1365-2222.2005.02315.x
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发表时间：
2005-09-01
影响因子：
6.1
通讯作者：
Björkstén, B
中科院分区：
文献类型：
--
作者：
Sepp, E;Julge, K;Björkstén, B
背景 在过去的几十年里，来自世界不同地区的几项研究表明过敏性疾病的患病率不断增加。这与环境因素有关，例如微生物压力的变化。我们之前的研究已经证明了过敏和非过敏儿童之间肠道微生物群的差异。目的 验证过敏和非过敏 5 岁爱沙尼亚儿童肠道微生物群和 IgE 反应相关的假设。方法 研究组由 19 名过敏儿童和 19 名非过敏 5 岁儿童组成，这些儿童是从出生起就进行跟踪的较大群体中选出的。过敏的诊断基于儿童的临床检查和问卷调查数据。通过在九种不同的培养基上接种系列稀释液并在不同的环境中孵育来量化粪便微生物群。肠道微生物群的组成以不同物种的绝对计数及其在已识别微生物群总数中的相对份额来表示。结果与健康儿童相比，患有过敏性疾病的儿童中双歧杆菌的检出率较低，而梭状芽胞杆菌在肠道微生物中所占的比例较高。具有针对特定过敏原的特异性 IgE 抗体的儿童具有较高的梭菌计数，并且梭菌计数与血清 IgE 水平相关，但仅在过敏儿童中如此。在非过敏儿童中，血清IgE水平与类杆菌计数呈正相关。结论过敏性疾病的发生似乎与肠道微生物生态系统的组成有关。大量潜在病原体（例如梭状芽胞杆菌）与过敏的临床表现和 IgE 抗体形成相关。
Background Over the last few decades, several studies from different parts of the world have indicated an increasing prevalence of allergic diseases. This has been related to environmental factors, like changes of microbial pressure. Our previous studies have demonstrated differences in the intestinal microbiota between allergic and non-allergic children.Aim To test the hypothesis that the intestinal microbiota and IgE response are related, both in allergic and non-allergic 5-year-old Estonian children.Methods The study group comprised 19 allergic and 19 non-allergic 5-year-old children, selected from a larger group who had been followed from birth. The diagnosis of allergy was based on clinical examination of the children and on data obtained from the questionnaires. The faecal microbiota were quantified by seeding serial dilutions on nine different media for incubation in different environment. The composition of the gut microbiota was expressed both as absolute counts of the various species and their relative share among the total counts of identified microbiota.Results Bifidobacteria were less commonly detected in children with allergic diseases than in healthy children and clostridia comprised a higher proportion among their gut microbes. Children with specific IgE antibodies to defined allergens had higher counts of clostridia and the counts of clostridia correlated with the level of serum IgE, but only so in allergic children. In non-allergic children, the serum IgE levels showed a positive correlation with the counts of bacteroides.Conclusion The development of allergic diseases seems to be associated with the composition of the gut microbial ecosystem. High counts of potential pathogens, such as clostridia, are associated with clinical manifestations of allergy and IgE antibody formation.