Estimation of nitric oxide production and reaction rates in tissue by use of a mathematical model
Estimation of nitric oxide production and reaction rates in tissue by use of a mathematical model
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DOI：
10.1152/ajpheart.1998.274.6.h2163
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发表时间：
1998-06-01
影响因子：
4.8
通讯作者：
Liao, JC
中科院分区：
文献类型：
--
作者：
Vaughn, MW;Kuo, L;Liao, JC
一氧化氮（NO）由血管内皮产生，是调节血管张力和通透性，抑制血小板粘附和聚集的重要生物信使。一氧化氮通过与血管平滑肌中的胍基环化酶相互作用，引发一系列导致血管扩张的反应，从而控制血管张力。由于缺乏NO的产生和降解速率的信息，以前通过NO扩散和反应的数学模型来研究这种相互作用的努力受到了阻碍。我们使用数学模型和先前发表的实验数据来估计NO的生成速率，6.8 x 10(-14) mu mol.mu m(-2).s(-1)；NO扩散系数3300 μ m(2) s(-1)；血管平滑肌NO消耗速率系数为0.01 s(-1)（一级速率表达）或0.05 μ M-1。S(-1)（二阶速率表达式）。详细讨论了建模方法。它为模拟内皮细胞产生的一氧化氮和估计相关的物理参数提供了一个总体框架。
Nitric oxide (NO) produced by the vascular endothelium is an important biologic messenger that regulates vessel tone and permeability and inhibits platelet adhesion and aggregation. NO exerts its control of vessel tone by interacting with guanylyl cyclase in the vascular smooth muscle to initiate a series of reactions that lead to vessel dilation. Previous efforts to investigate this interaction by mathematical modeling of NO diffusion and reaction have been hampered by the lack of information on the production and degradation rate of NO. We use a mathematical model and previously published experimental data to estimate the rate of NO production, 6.8 x 10(-14) mu mol.mu m(-2).s(-1); the NO diffusion coefficient, 3,300 mu m(2) s(-1); and the NO consumption rate coefficient in the vascular smooth muscle, 0.01 s(-1) (Ist-order rate expression) or 0.05 mu M-1.s(-1) (2nd-order rate expression). The modeling approach is discussed in detail. It provides a general framework for modeling the NO produced from the endothelium and for estimating relevant physical parameters.