EFFECT OF OZONE INHALATION ON THE RESPONSE TO NASAL CHALLENGE WITH ANTIGEN OF ALLERGIC SUBJECTS
EFFECT OF OZONE INHALATION ON THE RESPONSE TO NASAL CHALLENGE WITH ANTIGEN OF ALLERGIC SUBJECTS
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DOI：
10.1164/ajrccm/142.3.594
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发表时间：
1990-09-01
期刊：
影响因子：
--
通讯作者：
PROUD, D
中科院分区：
文献类型：
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作者：
BASCOM, R;NACLERIO, RM;PROUD, D
氧化剂吸入对过敏性疾病的影响是令人感兴趣的，因为过敏性患者经常报告在空气质量差的情况下呼吸道症状增加，并且药理学研究表明空气污染物臭氧水平增加与哮喘恶化之间存在关联。本研究的目的是描述无症状的过敏性受试者吸入臭氧后的上呼吸道反应，并确定臭氧预暴露是否增加了这些受试者对抗原鼻腔激发的急性反应。一组12名无症状受试者过敏性鼻炎的历史，暴露在一个随机的，交叉设计，在休息，在每2天，分别为2周，4小时的清洁空气或0.5 ppm臭氧的环境室。暴露期后，受试者接受4剂抗原（1至1，000 PNU豚草或草）的鼻激发。每次给药后对症状进行评级并进行鼻灌洗。 测量组胺和白蛋白浓度和TAME-酯酶活性，并测定细胞计数和分化。暴露于臭氧引起上呼吸道和下呼吸道症状显著增加，混合炎性细胞流入，海军灌洗液中性粒细胞增加7倍，嗜酸性粒细胞增加20倍，单核细胞增加10倍，以及上皮细胞明显脱落。在臭氧和清洁空气暴露日，鼻灌洗液白蛋白浓度显著增加。TAME酯酶活性总体上没有显著增加，但在12名受试者中的5名中至少增加了两倍。对随后用抗原激发的反应针对臭氧后值进行标准化。抗原诱导的上呼吸道和下呼吸道症状，细胞和介质的差异与臭氧相比，清洁空气暴露。结果表明无症状过敏受试者对臭氧的上呼吸道炎症反应，但在此水平、持续时间和臭氧暴露时间下，对抗原鼻腔激发的急性反应没有改变。
The effect of oxidant inhalation on allergic illness is of interest because allergic patients often report increased respiratory symptoms during episodes of poor air quality, and epidemilogic studies demonstrate an association between increased levels of the air pollutant ozone and exacerbations of asthma. The purpose of this study was to characterize the upper respiratory response to ozone inhalation in asymptomatic, allergic subjects and to determine whether ozone preexposure increased the acute response to nasal challenge with antigen in these subjects. A group of 12 asymptomatic subjects with a history of allergic rhinitis were exposed in a randomized, cross-over design, at rest, on each of 2 days, separately by 2 wk, to 4 h of clean air or 0.5 ppm ozone in an environmental chamber. Following the exposure period, subjects underwent nasal challenge with four doses of antigen (1 to 1,000 PNU ragweed or grass). Symptoms were rated and nasal lavage performed after each dose. Measurement of histamine and albumin concentration and TAME-esterase activity and determination of cell counts and differentials were performed. Exposure to ozone caused significant increases in upper and lower respiratory symptoms, a mixed inflammatory cell influx with a sevenfold increase in naval lavage neutrophils, a 20-fold increase in eosinophils, and a tenfold increase in mononuclear cells, as well as an apparent sloughing of epithelial cells. There was a significant increase in nasal lavage albumin concentration on the ozone and clean air exposure days. TAME-esterase activity showed no significant increase overall, but increased at least twofold in 5 of 12 subjects. The response to subsequent challenge with antigen was normalized for the post-ozone values. Antigen-induced differences in upper and lower respiratory symptoms, cells, and mediators were all equivalent with ozone compared to clean air exposure. The results demonstrate an upper respiratory inflammatory response to ozone in asymptomatic allergic subjects but demonstrate no alteration of the acute response to nasal challenge with antigen at this level, duration, and timing of ozone exposure.