Fabrication and effect on regulating vSMC phenotype of a biomimetic tunica media scaffold
Fabrication and effect on regulating vSMC phenotype of a biomimetic tunica media scaffold
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仿生中膜支架的制备及其对vSMC表型的调节作用
DOI：
10.1039/c6tb02437h
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发表时间：
2016
影响因子：
7
通讯作者：
Wu Decheng
中科院分区：
文献类型：
--
作者：
Shen Hong;Hu Xixue;Cui Haiyan;Zhuang Yaping;Huang Da;Yang Fei;Wang Xing;Wang Shenguo;Wu Decheng
理想的血管组织工程支架应模仿天然血管中的物理和生化线索，以指导细胞生长、分化和组织形成。中膜为原生血管提供了关键的结构和功能支持。本研究采用软光刻技术结合溶液铸造和相分离技术，制备了模拟天然血管中膜介质物理生化线索的膜状MNP-TGF/bFGF-PLGA支架。支架具有平行排列的微沟槽和纳米纤维结构的双重表面形貌，并且具有相互连接的孔，能够输送营养物质并消除代谢产物。通过纳米二氧化硅结合和等离子体处理技术将TGF-β1和bFGF固定在支架上，分别保持10天和7天的持续释放。双重表面形貌和释放的生长因子的协同作用使MNP-TGF/bFGF-PLGA支架具有良好的调节血管平滑肌细胞（vSMC）表型的能力。重要的是，该支架具有良好的力学性能，可以很容易地卷成多层圆柱形管，是一种很有前途的仿生血管组织工程支架。
An ideal vascular tissue engineering scaffold should imitate physical and biochemical cues in native vessels for guiding cell growth, differentiation and tissue formation. The tunica media provides a key structure and function support for native vessels. In this study, a film-like MNP-TGF/bFGF-PLGA scaffold that simulated physical and biochemical cues of tunica media in native vessels was fabricated by soft lithography combined with solution casting and phase separation technique. The scaffold had dual surface topographies of parallel arranged microgrooves and nanofiber structures, and interconnected pores to be able to deliver nutrient and eliminate metabolized products. The TGF-β1 and bFGF immobilized on the scaffold by silica nanoparticle binding and plasma treatment technique could maintain continuous release for 10 and 7 days, respectively. The synergy effect of the dual surface topography and released growth factors endowed the MNP-TGF/bFGF-PLGA scaffold with good capacity on regulating vascular smooth muscle cell (vSMC) phenotype. Importantly, the scaffold possessed good mechanical properties and could easily be rolled into a multilayer cylindrical tube as a promising biomimic vascular tissue engineering scaffold.