CELL-AUTONOMOUS ACTION OF ZEBRAFISH SPT-1 MUTATION IN SPECIFIC MESODERMAL PRECURSORS
CELL-AUTONOMOUS ACTION OF ZEBRAFISH SPT-1 MUTATION IN SPECIFIC MESODERMAL PRECURSORS
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DOI：
10.1038/348728a0
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发表时间：
1990-12-20
期刊：
影响因子：
64.8
通讯作者：
KANE, DA
中科院分区：
文献类型：
--
作者：
HO, RK;KANE, DA
在斑马鱼中，就像爪蟾一样，原肠胚形成过程中精心策划的细胞运动可以分解为几个部分，包括外延2、内卷3、4、收敛和延伸4。隐性致死突变 pt-1(b104) 或“铲尾”纯合的胚胎在胚胎躯干中具有一组复杂的缺陷，这些缺陷可能在失去这些运动之一后继发出现，这些运动通常会产生躯干体节中胚层 5（图 1）。现在，我们通过在野生型和突变型胚胎之间移植细胞来验证这一假设，以确定spt-1直接影响的细胞。我们的结果表明，突变仅自主影响位于早期原肠胚胚盘侧缘的中胚层前体6。其他中胚层细胞和所有外胚层前体似乎不需要野生型基因的功能。我们的研究结果揭示了脊椎动物原肠胚形成的出人意料的微妙遗传控制。
IN zebrafish, as inXenopus1the well-orchestrated cell movements of gastrulation can be dissected into several components, including epiboly2, involution3,4, convergence and extension4. Embryos homozygous for the recessive lethal mutationspt-l(b104)or 'spadetail' have a complex set of defects in the trunk of the embryo that may arise secondarily after loss of one of these movements, convergence, from those precursors that would normally have given rise to trunk somitic mesoderm5(Fig. 1). We have now tested this hypothesis by transplanting cells between wild-type and mutant embryos, to identify the cells thatspt-1affects directly. Our results show that the mutation autonomously affects only those mesodermal precursors located along the lateral margin of the early gastrula blastoderm6. Other mesodermal cells and all ectodermal precursors seem not to require function of the wild-type gene. Our findings reveal an unexpectedly delicate genetic control of vertebrate gastrulation.