Multifunctional Magnetoplasmonic Nanoparticle Assemblies for Cancer Therapy and Diagnostics (Theranostics)
Multifunctional Magnetoplasmonic Nanoparticle Assemblies for Cancer Therapy and Diagnostics (Theranostics)
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DOI：
10.1002/marc.200900793
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发表时间：
2010-01-18
影响因子：
4.6
通讯作者：
Kotov, Nicholas A.
中科院分区：
文献类型：
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作者：
Chen, Wei;Xu, Naifeng;Kotov, Nicholas A.
在这项工作中，我们描述了具有磁浆性质的复杂纳米粒子组件的制备和生物医学功能，该组件适合同时用于癌症治疗和诊断(治疗)。最常见的磁浆纳米结构是通过仔细调整金属还原方案而制成的，这既繁琐又有限制性。在这里，我们应用纳米级组装的策略来从单个构建块制备这样的系统。所制备的超结构是基于包裹在二氧化硅外壳中的磁性Fe3O4纳米颗粒，代表磁性模块。核心被代表等离子体模块的金纳米颗粒以电晕状的方式包围。作为额外的功能，它们还被聚乙二醇链包裹，作为隐蔽剂，以延长血液循环时间。准备工作异常简单，并允许人们改变每个功能的贡献。这两个模块都可以携带药物，在本研究中，它们装载了潜在的抗癌药物姜黄素。采用显微镜、光谱分析和生物化学等多种方法，研究了纳米粒子组装体对白血病HL-60细胞的成像和治疗作用。高对比度磁共振图像和高凋亡率证明了组装法制备磁等离子纳米粒子的成功。这项技术使人们能够轻松地“拨入”个性化治疗方案的临床设置的功能。
In this work, we describe the preparation and biomedical functionalities of complex nanoparticle assemblies with magnetoplasmonic properties suitable for simultaneous cancer therapy and diagnostics (theranostics). Most commonly magnetoplasmonic nanostructures are made by careful adaptation of metal reduction protocols which is both tedious and restrictive. Here we apply the strategy of nanoscale assemblies to prepare such systems from individual building blocks. The prepared superstructures are based on magnetic Fe3O4 nanoparticles encapsulated in silica shell representing the magnetic module. The cores are surrounded in a corona-like fashion by gold nanoparticles representing the plasmonic module. As additional functionality they were also coated by poly(ethyleneglycol) chains as a cloaking agent to extend the blood circulation time. The preparation is exceptionally simple and allows one to vary the contribution of each function. Both modules can carry drugs and, in this study, they were loaded with the potential anticancer drug curcumin. A comprehensive set of microscopy, spectroscopy and biochemical methods were applied to characterize both imaging and therapeutic function of the nanoparticle assemblies against leukemia HL-60 cells. High contrast magnetic resonance images and high apoptosis rates demonstrate the success of assembly approach for the preparation of magnetoplasmonic nanoparticles. This technology allows one to easily "dial in" the functionalities in the clinical setting for personalized theranostic regiments.