Stability and Antibiofilm Efficiency of Slightly Acidic Electrolyzed Water Against Mixed-Species of Listeria monocytogenes and Staphylococcus aureus.
Stability and Antibiofilm Efficiency of Slightly Acidic Electrolyzed Water Against Mixed-Species of Listeria monocytogenes and Staphylococcus aureus.
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DOI：
10.3389/fmicb.2022.865918
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发表时间：
2022
影响因子：
5.2
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中科院分区：
文献类型：
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在自然环境中，大多数微生物生活在混合物种生物膜中，生物体在混合物种生物膜中的代谢和生长不同于单一物种生物膜。粘附在食品加工设备表面的细菌及其生物膜是交叉污染的来源，对人类造成风险。微酸性电解水（SAEW）已被提议作为食品和农业工业中的新型消毒剂。本文研究了不同条件下SAEW物理性质的变化，以及SAEW对产芽孢和非产芽孢病原菌的消毒能力。此外，我们研究了消毒能力的SAEW后，12个月的货架寿命上的混合物种的生物膜单核细胞增生李斯特菌斯科特A和金黄色葡萄球菌。结果表明，30和50 ppm的SAEW在30 s内对产孢病原菌蜡样芽孢杆菌（Bacilluscereus）全部杀灭。生产的SAEW的ACC和pH值的变化一般受储存条件的影响。在整个储存期间，在HDPE封闭条件下，用50 ppm SAEW处理5 min，未检测到孢子形成和非孢子形成病原体。25 mg/L的SAEW能有效地抑制L.单核细胞增多症Scott A和S.在5分钟处理内，生物膜中的金黄色葡萄球菌生物膜细胞为~2.45和2.57 log CFU/mL。然而，两种细菌在混合物种生物膜中的下降分别为1.95和1.43 log CFU/mL。利用原子力显微镜和激光共聚焦显微镜观察了SAEW处理后混合生物膜细胞膜通透性的变化。L.在混合种生物膜细胞中，单核细胞增多症Scott A对SAEW更敏感。结果表明，SAEW具有较强的破膜活性，能破坏生物膜细胞膜，降低细胞密度，消除生物膜，是一种优良的食品加工抗菌剂。
In the natural environment, most microorganisms live in mixed-species biofilms, in which the metabolism and growth of organisms are different from that in single-species biofilms. Adhesive bacteria and their biofilms on the surface of food processing equipment are the sources of cross-contamination, leading to the risk for humans. Slightly acidic electrolyzed water (SAEW) has been proposed as a novel sanitizer in the food and agriculture industry. In this study, we investigated the changes in the physical properties of SAEW under different conditions and the disinfection abilities of SAEW against spore-forming and non-spore-forming pathogens. Furthermore, we examined the disinfection abilities of SAEW after 12 months of shelf life on a mixed-species biofilm of Listeria monocytogenes Scott A and Staphylococcus aureus. The results showed that SAEW at 30 and 50 ppm achieved all-kill of the spore-forming pathogen Bacillus cereus within 30 s. Changes in the ACC and pH of the produced SAEW were generally affected by the storage conditions. Both spore-forming and non-spore-forming pathogens were not detected under treatment with 50 ppm SAEW for 5 min under HDPE-closed conditions throughout the whole storage period. Moreover, 25 mg/L SAEW can inactivate L. monocytogenes Scott A and S. aureus biofilm cells in ~2.45 and 2.57 log CFU/mL in biofilms within 5-min treatment. However, the decline of the two bacteria in the mixed-species biofilm was 1.95 and 1.43 log CFU/mL, respectively. The changes in the cell membrane permeability of the mixed-species biofilm under treatment with SAEW were observed by using atomic force microscopy and confocal laser scanning microscopy. L. monocytogenes Scott A was more sensitive to SAEW in the mixed-species biofilm cells. These findings exhibited strong antibiofilm activities of SAEW in impairing biofilm cell membranes, decreasing cell density, and eliminating biofilm, which suggest that SAEW is an excellent antibacterial agent in the food processing industries.