Green synthesis of silver nanoparticles using Carum copticum: Assessment of its quorum sensing and biofilm inhibitory potential against gram negative bacterial pathogens
Green synthesis of silver nanoparticles using Carum copticum: Assessment of its quorum sensing and biofilm inhibitory potential against gram negative bacterial pathogens
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DOI：
10.1016/j.micpath.2020.104172
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发表时间：
2020-07-01
影响因子：
3.8
通讯作者：
Hassan, Iftekhar
中科院分区：
文献类型：
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作者：
Abul Qais, Faizan;Shafiq, Anam;Hassan, Iftekhar
病原菌的耐药性已成为全球性的人类健康威胁。由于新的抗微生物药物的开发进展缓慢，因此需要开发新的替代策略来对抗多药耐药性问题。此外，人们关注的是用于合成具有有效药用特性（包括抗毒力特性）的纳米颗粒的生态友好方法，以解决多药耐药性的出现。靶向群体感应控制的毒力因子和生物膜已成为抗感染药物的新靶点。以黄连水提物为原料制备了银纳米粒子（Ag@CC-NPs），并采用紫外-可见吸收光谱、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、透射电子显微镜（TEM）和扫描电子显微镜（SEM）对其进行了表征。检查Ag@ CC-NP在亚MIC值下抑制群体感应介导的毒力因子和生物膜对抗三种测试病原体的能力。Ag@CC-NPs对C.紫罗兰。铜绿假单胞菌毒力因子如绿脓菌素产生、绿脓菌荧光素产生、外切蛋白酶活性、弹性蛋白酶活性、游泳运动性和鼠李糖脂产生在亚MIC下被抑制76.9%、49.0%、71.1%、53.3%、89.5%和60.0%。此外，S.粘质虫即灵菌红素的产生、外切蛋白酶活性和群集运动性降低了78.4、67.8和90.7%。Ag@CC-NPs还表现出广谱的生物膜活性，对铜绿假单胞菌、S. marcescens和C. violaceum分别。从SEM和CLSM分析可以看出，玻璃盖玻片上的生物膜形成显著减少。这些发现揭示了Ag@ CC-NP对细菌病原体的体外功效，并且可以用于开发用于局部应用的细菌感染管理（主要是伤口感染）或表面涂层以防止细菌粘附在医疗器械上的替代治疗剂。
Antimicrobial resistance among pathogenic bacteria has become a global threat to human health. Due to poor progress in development of new antimicrobial drugs, there is a need for the development of novel alternative strategies to combat the problem of multidrug resistance. Moreover, there is focus on ecofriendly approach for the synthesis nanoparticles having efficient medicinal properties including antivirulence properties to tackle the emergence of multi-drug resistance. Targeting quorum sensing controlled virulence factors and biofilms has come out to be a novel anti-infective drug target. The silver nanoparticles (Ag@CC-NPs) were synthesized from aqueous extract of Carum copticum and characterized using UV-vis absorption spectroscopy, fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Ag@CC-NPs were checked for its ability to inhibit quorum sensing-mediated virulence factors and biofilms against three test pathogens at sub-MIC values. There was similar to 75% inhibition of violacein production by Ag@CC-NPs against C. violaceum. The P. aeruginosa virulence factors such as pyocyanin production, pyoverdin production, exoprotease activity, elastase activity, swimming motility and rhamnolipid production were inhibited by 76.9, 49.0, 71.1, 53.3, 89.5, and 60.0% at sub-MIC. Moreover, virulence factors of S. marcescens viz. prodigiosin production, exoprotease activity, and swarming motility was reduced by 78.4, 67.8, and 90.7%. Ag@CC-NPs also exhibited broad-spectrum antibiofilm activity with 77.6, 86.3, and 75.1% inhibition of biofilms of P. aeruginosa, S. marcescens, and C. violaceum respectively. The biofilm formation on glass coverslip was reduced remarkably as evident from SEM and CLSM analysis. The findings revealed the in vitro efficacy of Ag@CC-NPs against bacterial pathogens and can be exploited in the development of alternative therapeutic agent in management of bacterial infections for topical application, mainly wound infection, or coating of surfaces to prevent bacterial adherence on medical devices.