Synthesis and characterization of Reishi mushroom-mediated green synthesis of silver nanoparticles for the biochemical applications
Synthesis and characterization of Reishi mushroom-mediated green synthesis of silver nanoparticles for the biochemical applications
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DOI：
10.1016/j.jpba.2019.112970
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发表时间：
2020-01-30
影响因子：
3.4
通讯作者：
Sen, Fatih
中科院分区：
文献类型：
--
作者：
Aygun, Aysenur;Ozdemir, Sadin;Sen, Fatih
近年来，通过生物方法合成纳米颗粒引起了相当大的关注。植物和植物提取物由于其丰富的生物活性代谢物而成为生物合成的最优选方法之一。在这项研究中，银纳米粒子（银纳米粒子）的合成使用灵芝（灵芝）提取物。不同的分析技术，包括X射线光电子能谱（XPS），X射线衍射（XRD），透射电子显微镜（TEM），紫外-可见光谱，和傅里叶变换红外光谱仪（FTIR）用于表征银纳米粒子。紫外-可见光谱在400-460 nm之间表现出宽吸收峰，表明银纳米颗粒的存在。TEM图像显示Ag纳米颗粒是球形的，直径范围为15-22 nm。此外，根据XRD表征技术，Ag纳米颗粒形成面心立方结构。并研究了该化合物对1-二苯基-2-苦肼基（DPPH）的抗氧化活性。250 mg/L时，DPPH自由基清除率最高，为76.45%。DNA切割活性结果表明，绿色Ag NPs分别在50和100 mg/L下引起单菌株DNA切割活性30和60 min。还研究了Ag NPs的抗微生物活性，并将结果记录为最小抑制浓度（MIC）。纳米银对革兰氏阳性菌（S。金黄色葡萄球菌E.你好，B。蜡样芽胞杆菌（E. cereus）和革兰氏阴性芽胞杆菌（E. coli、铜绿假单胞菌、L.嗜肺菌亚种嗜肺菌属）细菌。此外，银纳米颗粒还显示出对C.白色真菌(C)2019 Elsevier B. V.版权所有。
In recent years, the synthesis of nanoparticles via biological processes has attracted considerable attention. The use of plants and plant extracts is one of the most preferred methods for biological synthesis due to their rich biologically active metabolites. In this study, silver nanoparticles (Ag NPs) were synthesized using reishi mushroom (Ganoderma lucidum) extract. Different analytical techniques including X-ray Photoelectron Spectroscopy (XPS), X-ray diffraction (XRD), transmission electron microscopy (TEM), UV-vis spectroscopy, and Fourier Transform Infrared Spectrophotometer (FTIR) were used for the characterization of Ag NPs. UV-vis spectrum exhibited a broad absorption peak between 400-460 nm which indicates the existence of Ag NPs. TEM images showed Ag NPs are spherical with a diameter range of 15-22 nm. In addition, it is shown that Ag NPs form a face-centered cubic structure according to XRD characterization technique. The antioxidant activity towards to 1-Diphenyl-2-picrylhydrazyl (DPPH) was also studied. The highest DPPH scavenging percentage was recorded as 76.45% at 250 mg/L. The DNA cleavage activity results indicated that the green Ag NPs caused single strain DNA cleavage activity for 30 and 60 min at 50 and 100 mg/L, respectively. The Ag NPs antimicrobial activity was also investigated and results recorded as minimum inhibition concentration (MIC). Ag NPs showed a strong antibacterial effect against gram-positive (S. aureus, E. hirae, B. cereus) and gram-negative (E. coli, P. aeruginosa, L. pneumophila subsp. Pneumophila) bacteria. Furthermore, Ag NPs have also been shown to have a high antifungal effect against C. albicans fungus. (C) 2019 Elsevier B.V. All rights reserved.