INVIVO LYMPHOCYTE-MEDIATED MYOCARDIAL INJURIES DEMONSTRATED BY ADOPTIVE TRANSFER OF EXPERIMENTAL AUTOIMMUNE MYOCARDITIS
INVIVO LYMPHOCYTE-MEDIATED MYOCARDIAL INJURIES DEMONSTRATED BY ADOPTIVE TRANSFER OF EXPERIMENTAL AUTOIMMUNE MYOCARDITIS
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DOI：
10.1161/01.cir.85.5.1918
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发表时间：
1992-05-01
期刊：
影响因子：
37.8
通讯作者：
FUJIWARA, M
中科院分区：
文献类型：
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作者：
KODAMA, M;MATSUMOTO, Y;FUJIWARA, M
背景。 为了阐明免疫相关心肌损伤的机制，我们检查了自身免疫性心肌炎是否可以通过体液或细胞因素被动转移。方法和结果。 通过用弗氏完全佐剂中的人心肌肌球蛋白组分进行免疫，在Lewis大鼠中诱发活动性心肌炎。 这种实验性心肌炎的特征是肉眼可见的特征，例如心包积液、心脏增大和心脏表面灰色变色。 组织学上观察到广泛的心肌坏死和大量炎性细胞浸润。 有趣的是，在病变中经常观察到多核巨细胞。 通过使用患有严重心肌炎的大鼠的新鲜血清和混合血清的免疫球蛋白级分来检查由体液因素引起的疾病的转移，并且通过使用来自患病大鼠的脾细胞和淋巴结细胞来检查由细胞因素引起的疾病的转移。 当幼稚Lewis大鼠被给予15.75毫克免疫球蛋白级分时，心脏中没有观察到特殊的变化。 新鲜血清也不能引起受体大鼠的心肌炎。 相反，静脉注射在1μg/ml伴刀豆球蛋白A存在下培养3天的脾细胞或淋巴结细胞会引发严重的心肌炎。 被动转移性心肌炎的宏观和微观表现与主动诱发的心肌炎基本相同。 转移性心肌炎病灶内也可见到多核巨细胞。结论。 这项研究证明了体内淋巴细胞介导的心肌损伤的直接证据。
Background. To elucidate the mechanisms of immune-related myocardial injuries, we examined whether autoimmune myocarditis was passively transferable by use of humoral or cellular factors.Methods and Results. Active myocarditis was elicited in Lewis rats by immunization with human cardiac myosin fraction in complete Freund's adjuvant. This experimental myocarditis was characterized by macroscopic features such as pericardial effusion, enlargement of the heart, and gray discoloration of the cardiac surface. Histologically, extensive myocardial necrosis and numerous inflammatory cell infiltrations were observed. Interestingly, multinucleated giant cells were frequently observed in the lesions. Transfer of the disease by the humoral factor was examined by use of fresh sera and immunoglobulin fraction of pooled sera from rats with severe myocarditis, and transfer by the cellular factor was tested by use of spleen cells and lymph node cells from the diseased rats. When naive Lewis rats were given 15.75 mg of immunoglobulin fraction, no particular change was observed in the hearts. Fresh sera also could not elicit myocarditis in recipient rats. In contrast, intravenous injection of spleen cells or lymph node cells that were cultured for 3 days in the presence of 1-mu-g/ml of concanavalin A elicited severe myocarditis. The macroscopic and microscopic findings of passively transferred myocarditis are essentially the same as those found in actively induced myocarditis. Multinucleated giant cells were also observed in the lesions of transferred myocarditis.Conclusions. This study demonstrates direct evidence for in vivo lymphocyte-mediated myocardial injuries.