Detection of C-Reactive Protein in Evanescent Wave Field Using Microparticle-Tracking Velocimetry
Detection of C-Reactive Protein in Evanescent Wave Field Using Microparticle-Tracking Velocimetry
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DOI：
10.1021/la102137j
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发表时间：
2010-09-07
期刊：
影响因子：
3.9
通讯作者：
Lin, Shiming
中科院分区：
文献类型：
--
作者：
Fan, Yu-Jui;Sheen, Horn-Jiunn;Lin, Shiming
提出了一种利用微粒子跟踪测速技术（micro-PTV）在瞬变波场中测量纳米粒子布朗运动的新方法，该方法可以为纳米粒子分析提供高信噪比的图像。运动。该方法可以实时检测c反应蛋白（CRP）与抗CRP包被纳米球之间的快速相互作用，因为CRP浓度与纳米球在平衡状态下的布朗速度有关。本实验采用最小的可观察纳米球，直径为185 nm，可检测低至0.1 μ g/mL的CRP浓度。即使在高粘度溶液中。此外，解离常数K-D可以根据实验结果进行计算。
A new technique is developed to measure the nanoparticles' Brownian motions by employing microparticle-tracking velocimetry (micro-PTV) in evanescent wave field, which can provide high signal-to-noise ratio images for analyzing nanoparticles. movements. This method enables real-time detection of C-reactive proteins (CRPs) during the rapid interaction between CRPs and anti-CRP-coated nanobeads as CRP concentrations are related to the nanobeads' Brownian velocity in the equilibrium state. The smallest observable nanobeads with 185 nm were utilized in this experiment to detect CRP concentrations as low as 0.1 mu g/mL. even in a high-viscosity solution. Further, the dissociation constant, K-D, can be evaluated based on the experimental results.