Differential expression of 70-kDa heat shock-protein in human oral tumorigenesis
Differential expression of 70-kDa heat shock-protein in human oral tumorigenesis
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DOI：
10.1002/ijc.2910630604
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发表时间：
1995-12-11
影响因子：
6.4
通讯作者：
Ralhan, R
中科院分区：
文献类型：
--
作者：
Kaur, J;Ralhan, R
为了了解口腔肿瘤发生多步骤过程背后的生物学事件，我们研究了人类正常、癌前和恶性口腔组织中 70-kDa 热休克蛋白 HSP70 的表达。使用针对 HSP70 的小鼠单克隆抗体，通过免疫染色和免疫印迹分析评估口腔组织样本中 HSP70 的表达。在口腔鳞状细胞癌中观察到强烈的细胞核和细胞质 HSP70 免疫染色 (30/38)。在口腔发育不良病变中观察到轻度至中度HSP70表达（19/30），在正常口腔组织中观察到基础低水平的HSP70。结果通过免疫印迹分析得到证实。 HSP70在正常、恶变前和恶性口腔病变中表达的巨大差异表明，它在口腔癌发生过程中存在差异表达，并且可能与肿瘤的发展有关。 (C) 1995 Wiley-Liss, Inc.
To understand the biological events underlying the multistep process of oral tumorigenesis we have studied the expression of 70-kDa heat-shock protein, HSP70, in human normal, premalignant and malignant oral tissues. Expression of HSP70 was assessed in oral-tissue specimens using a mouse monoclonal antibody against HSP70 by immunostaining and immunoblotting analysis. Strong nuclear and cytoplasmic HSP70 immunostaining was observed in oral squamous-cell carcinomas (30/38). Mild to moderate HSP70 expression was observed in oral dysplastic lesions (19/30) and basal low level of HSP70 was observed in normal oral tissues. The results were corroborated by immunoblotting analysis. The wide variation in HSP70 expression in normal, pre-malignant and malignant oral lesions suggests that it is differentially expressed during oral carcinogenesis and may be implicated in tumor development. (C) 1995 Wiley-Liss, Inc.