Gold nanorods enable noninvasive longitudinal monitoring of hydrogels in vivo with photoacoustic tomography.
Gold nanorods enable noninvasive longitudinal monitoring of hydrogels in vivo with photoacoustic tomography.
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DOI：
10.1016/j.actbio.2020.09.048
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发表时间：
2020-09
影响因子：
9.7
通讯作者：
B. Shrestha;Katerina Stojkova;Richard C. Yi;M. Anastasio;J. Ye;E. Brey
中科院分区：
文献类型：
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作者：
B. Shrestha;Katerina Stojkova;Richard C. Yi;M. Anastasio;J. Ye;E. Brey
纵向体内监测对于生物材料的设计和评估至关重要。理想的方法应提供三维定量信息、高空间分辨率、深层组织穿透以及组织和材料结构之间的对比度。光声 (PA) 或光声成像是一种混合技术，可实现高空间分辨率的三维成像。此外，光声成像可以根据血红蛋白的固有对比度对血管形成进行成像。在这项研究中，我们研究了光声计算机断层扫描（PACT）作为纵向监测小动物模型中植入的水凝胶的工具。水凝胶中装有金纳米棒以增强对比度，并每周成像一次，持续 8 周。 PACT 允许在整个 8 周内对水凝胶进行非侵入性三维定量成像。采用定量体积分析来评估植入物的体内降解动力学，该动力学与体外预测略有偏差。多光谱成像可以同时分析水凝胶降解和局部血管化。这些结果支持了 PACT 作为洞察生物材料体内性能的工具的巨大潜力。
Longitudinalin vivomonitoring is essential for the design and evaluation of biomaterials. An ideal method would provide three-dimensional quantitative information, high spatial resolution, deep tissue penetration, and contrast between tissue and material structures. Photoacoustic (PA) or optoacoustic imaging is a hybrid technique that allows three-dimensional imaging with high spatial resolution. In addition, photoacoustic imaging allows for imaging of vascularization based on the intrinsic contrast of hemoglobin. In this study, we investigated photoacoustic computed tomography (PACT) as a tool for longitudinal monitoring of an implanted hydrogel in a small animal model. Hydrogels were loaded with gold nanorods to enhance contrast and imaged weekly for 8 weeks. PACT allowed non-invasive three-dimensional, quantitative imaging of the hydrogels over the entire 8 weeks. Quantitative volume analysis was used to evaluate thein vivodegradation kinetics of the implants which deviated slightly fromin vitropredictions. Multispectral imaging allowed for the simultaneous analysis of hydrogel degradation and local vascularization. These results provide support for the substantial potential of PACT as a tool for insight into biomaterial performancein vivo.