Dendritic cell inhibitory and activating immunoreceptors (DCIR and DCAR) in duck: Genomic organization and expression
Dendritic cell inhibitory and activating immunoreceptors (DCIR and DCAR) in duck: Genomic organization and expression
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DOI：
10.1016/j.molimm.2008.06.014
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发表时间：
2008-09-01
影响因子：
3.6
通讯作者：
Magor, Katharine E.
中科院分区：
文献类型：
--
作者：
Guo, Xiaoxin;Branton, William G.;Magor, Katharine E.
从鸭脾脏EST文库中克隆了编码DC抑制受体和DC激活受体的C型凝集素免疫受体基因DCIR和DCAR。这些受体因其作为抗原呈递细胞的调节剂的潜力而受到关注。分离基因组克隆并完全测序，其含有串联排列的一个DCIR基因和两个DCAR基因。鸭DCIR编码一种抑制性受体，其特征在于细胞质结构域中的基于免疫受体酪氨酸的抑制基序（ITIM）。DCAR1是一个假基因。DCAR2编码在跨膜区具有带正电荷残基的活化受体。DCIR和DCAR 2的全长和可变剪接形式是显而易见的。鸭DCIR和DCAR转录本优先在免疫和粘膜组织中表达，包括脾、法氏囊、肠和肺。靶向树突状细胞上的这些受体具有打破耐受性或增强免疫应答的潜力，这与B型肝炎的鸭模型和针对禽流感的疫苗接种有关。(C)2008爱思唯尔有限公司保留所有权利。
C-type lectin immunoreceptor genes encoding DC inhibitory and activating receptors (DCIR and DCAR) were identified in a spleen EST library of duck (Anas platyrhynchos). These receptors are of interest for their potential as regulators of antigen presenting cells. A genomic clone was isolated and fully sequenced, containing one DCIR gene and two DCAR genes arranged in tandem order. Duck DCIR encodes an inhibitory receptor that features an immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic domain. DCAR1 is a pseudogene. DCAR2 encodes an activating receptor with a positively charged residue in the transmembrane region. Full-length and alternatively spliced forms of both DCIR and DCAR2 are apparent. Duck DCIR and DCAR transcripts are preferentially expressed in immune and mucosal tissues including spleen, bursa of Fabricius, intestine and lung. Targeting these receptors on dendritic cells holds potential for breaking tolerance or for enhancing immune responses, relevant to the duck model for hepatitis B and vaccination against avian influenza. (C) 2008 Elsevier Ltd. All rights reserved.