Deep sequencing of the tilapia (Oreochromis niloticus) liver transcriptome response to dietary protein to starch ratio
Deep sequencing of the tilapia (Oreochromis niloticus) liver transcriptome response to dietary protein to starch ratio
复制标题
DOI：
10.1016/j.aquaculture.2014.06.009
复制
发表时间：
2014-09
期刊：
影响因子：
4.5
通讯作者：
Y. Xiong;Junfeng Huang;Xiaoxia Li;Liliu Zhou;Fang Dong;H. Ye;L. Gan
中科院分区：
文献类型：
--
作者：
Y. Xiong;Junfeng Huang;Xiaoxia Li;Liliu Zhou;Fang Dong;H. Ye;L. Gan
为了全面了解日粮蛋白质淀粉比对罗非鱼生理状态变化的影响，采用高蛋白淀粉比（HP，蛋白质含量为 33.5%，淀粉含量为 16.62%）和低蛋白质淀粉比等能量日粮（LP，蛋白质含量为 25.2%，淀粉含量为 26.82%）饲喂 8 周。我们的结果表明，用 LP 饲料喂养的鱼的生长性能和饲料利用率明显较差。较低的膳食蛋白质与淀粉比例还导致血浆胆固醇、血浆三酰甘油、肝脏脂质和肌肉脂质含量显着升高，这表明鱼体内脂肪沉积较多。采用 RNA-seq 评估罗非鱼肝脏转录组对 LP 饮食的反应。 RNA-seq数据显示，LP饮食有71个基因显着上调，26个基因显着下调。不同的表达基因被映射到47个信号通路，包括糖酵解、糖异生、氨基酸生物合成、脂肪生成和脂肪分解等。本研究全面了解了日粮蛋白质和淀粉比例对罗非鱼生理变化影响的分子机制。
To comprehensively understand the effects of dietary protein to starch ratio on alternations in physiologic status of tilapia, fish were fed with high protein to starch ratio (HP, 33.5% protein and 16.62% starch content) and low protein to starch ratio isoenergetic diets (LP, 25.2% protein and 26.82% starch content) for 8 weeks. Our results indicated that the fish fed with LP diet had a significantly poor growth performance and feed utilization. Lower dietary protein to starch ratio also resulted in markedly higher plasma cholesterol, plasma triacylglycerol, liver lipid and muscle lipid content, which indicated more fat deposition in fish. RNA-seq was employed to evaluate the tilapia hepatic transcriptome response to LP diet. RNA-seq data showed that 71 genes were significantly up-regulated and 26 genes were significantly down-regulated by LP diet. Different expression genes were mapped to 47 signaling pathways including glycolysis, gluconeogenesis, biosynthesis of amino acids, lipogenesis and lipolysis etc. The present study gains a comprehensive understanding of the molecular mechanisms underlying the effects of dietary protein and starch ratio on alternations in physiologic of tilapia.