Intestinal microbial communities associated with acute enteric infections and disease recovery.
Intestinal microbial communities associated with acute enteric infections and disease recovery.
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DOI：
10.1186/s40168-015-0109-2
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发表时间：
2015-09-22
期刊：
影响因子：
15.5
通讯作者：
Manning SD
中科院分区：
文献类型：
--
作者：
Singh P;Teal TK;Marsh TL;Tiedje JM;Mosci R;Jernigan K;Zell A;Newton DW;Salimnia H;Lephart P;Sundin D;Khalife W;Britton RA;Rudrik JT;Manning SD
肠道微生物组代表了一个复杂的微生物网络，对人类健康和防止病原体入侵至关重要。检查有和没有肠道感染的个体之间肠道微生物群落差异的研究有助于识别支持或阻碍肠道健康的微生物。对肠道感染患者（n = 200）和75名健康家庭成员的粪便DNA进行16 S rRNA基因测序，以确定肠道群落组成的差异。感染后还检查了13名患者的粪便，以更好地了解肠道群落如何恢复。患者社区有较低的物种丰富度，均匀度和多样性与未感染的社区，而主坐标分析表明未感染的社区，但不是患者社区，无论年龄，性别和种族的紧密聚类。患者和家庭成员之间的社区组成的差异主要是由于门变形菌门，拟杆菌门，厚壁菌门的丰度变化。患者社区有显着更多的变形杆菌属代表埃希氏菌相对于未感染的社区，这是占主导地位的拟杆菌。来自血性腹泻患者的肠道菌群在相邻连接群中聚集在一起，而来自13名患者感染后的菌群中拟杆菌门和厚壁菌门显著增加，并与未感染菌群聚集在一起。这些数据表明，肠道细菌感染患者的肠道菌群以类似的方式发生改变。此外，防止大肠杆菌丰度的增加可能是未来预防策略的重要考虑因素。本文的在线版本（doi：10.1186/s40168-015-0109-2）包含补充材料，可供授权用户使用。
The intestinal microbiome represents a complex network of microbes that are important for human health and preventing pathogen invasion. Studies that examine differences in intestinal microbial communities across individuals with and without enteric infections are useful for identifying microbes that support or impede intestinal health. 16S rRNA gene sequencing was conducted on stool DNA from patients with enteric infections (n = 200) and 75 healthy family members to identify differences in intestinal community composition. Stools from 13 patients were also examined post-infection to better understand how intestinal communities recover. Patient communities had lower species richness, evenness, and diversity versus uninfected communities, while principle coordinate analysis demonstrated close clustering of uninfected communities, but not the patient communities, irrespective of age, gender, and race. Differences in community composition between patients and family members were mostly due to variation in the abundance of phyla Proteobacteria, Bacteroidetes, and Firmicutes. Patient communities had significantly more Proteobacteria representing genus Escherichia relative to uninfected communities, which were dominated by Bacteroides. Intestinal communities from patients with bloody diarrhea clustered together in the neighbor-joining phylogeny, while communities from 13 patients’ post-infection had a significant increase in Bacteroidetes and Firmicutes and clustered together with uninfected communities. These data demonstrate that the intestinal communities in patients with enteric bacterial infections get altered in similar ways. Furthermore, preventing an increase in Escherichia abundance may be an important consideration for future prevention strategies. The online version of this article (doi:10.1186/s40168-015-0109-2) contains supplementary material, which is available to authorized users.