Autoclaving kills soil microbes yet soil enzymes remain active
Autoclaving kills soil microbes yet soil enzymes remain active
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DOI：
10.1016/j.pedobi.2007.05.002
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发表时间：
2007-01-01
期刊：
影响因子：
2.3
通讯作者：
Tibbett, Mark
中科院分区：
文献类型：
--
作者：
Carter, David O.;Yellowlees, David;Tibbett, Mark
杀生剂。土壤处理是用来去除或抑制土壤微生物的。因此，它提供了对土壤生物学和土壤过程之间关系的洞察。化学(土壤)。对来自澳大利亚昆士兰北部热带稀树草原生态系统的三种不同土壤的pH值、磷酸二酯酶和蛋白酶)和生物(底物诱导呼吸)特性进行了野外新鲜取样和连续两天的高压灭菌(121℃/103kPa30min)的测定。高压灭菌处理降低了土壤微生物生物量，并显著降低了三种土壤中的蛋白水解酶活性(接近90%)。高岭土中的磷酸二酯酶活性也显著降低了78%和92%。然而，高压灭菌处理的近晶土壤仅降低了4%的磷酸二酯酶活性。这项研究表明，磷酸二酯酶在极端条件下可以保持稳定。这可能是澳大利亚热带稀树草原生态系统中收缩膨胀粘土中磷循环的一个重要特征。(C)2007年爱思唯尔股份有限公司。版权所有。
Biocidal. treatment of soil is used to remove or inhibit soil microbial. activity, and thus provides insight into the relationship between soil biology and soil processes. Chemical (soil. pH, phosphodiesterase, protease) and biological (substrate induced respiration) characteristics of three contrasting soils from tropical savanna ecosystems in north Queensland, Australia were measured in field fresh samples and following autoclaving (121 degrees C/103 kPa for 30 min on two consecutive days). Autoclaving treatment killed the active soil microbial biomass and significantly decreased protease activity (similar to 90%) in all three soils. Phosphodiesterase activity in kaolinitic soils also significantly decreased by 78% and 92%. However, autoclave treatment of smectitic soil only decreased phosphodiesterase activity by 4% only. This study demonstrates phosphodiesterase can remain stable in extreme conditions. This might be a characteristic vital to the cycling of phosphorus in shrink-swell clays in Australian tropical savanna ecosystems. (c) 2007 Elsevier GmbH. All rights reserved.