Characterization of the promoter and extended C-terminal domain of Arabidopsis WRKY33 and functional analysis of tomato WRKY33 homologues in plant stress responses.
Characterization of the promoter and extended C-terminal domain of Arabidopsis WRKY33 and functional analysis of tomato WRKY33 homologues in plant stress responses.
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拟南芥WRKY33启动子和C端延伸结构域的表征以及番茄WRKY33同源物在植物胁迫反应中的功能分析
DOI：
10.1093/jxb/erv221
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发表时间：
2015-08
影响因子：
6.9
通讯作者：
Chen Z
中科院分区：
文献类型：
--
作者：
Zhou J;Wang J;Zheng Z;Fan B;Yu JQ;Chen Z
我们证明WRKY33蛋白在进化上是保守的，并在广泛的植物胁迫反应中发挥关键作用。c端结构域和特定启动子都参与wrky33诱导的胁迫耐受。拟南芥AtWRKY33在广泛的植物胁迫响应中起关键作用。AtWRKY33在其他植物中是否存在进化上保守的同源物，以及AtWRKY33在植物胁迫反应中如此重要的原因在很大程度上是未知的。我们将AtWRKY33与其近亲同源物进行比较，以确定AtWRKY33特异性的调控和结构元件，然后通过互补对其进行功能分析。我们还进行了系统发育分析，以确定AtWRKY33在其他植物中的结构同源物，并通过互补和基因沉默对两个番茄同源物进行了功能分析。AtWRKY33有一个扩展的c -末端结构域（CTD），这在它的近亲AtWRKY25中是不存在的。AtWRKY33的CTD和强烈的病原体/应激反应性表达都是补充AtWRKY33关键表型所必需的。AtWRKY33结构同源物在双子叶和单子叶植物中均有发现，其中在番茄中有两个同源物（SlWRKY33A和SlWRKY33B）。分子互补和基因沉默证实了两个番茄WRKY基因在植物逆境响应中发挥着与AtWRKY33相似的关键作用。因此，WRKY33蛋白在进化上是保守的，在广泛的植物胁迫反应中起着关键作用。WRKY33的CTD和启动子对于WRKY33在植物胁迫反应中发挥独特的重要作用至关重要。
We demonstrate that WRKY33 proteins are evolutionarily conserved and play a critical role in broad plant stress responses. Both C-terminal domain and specific promoter are involved in WRKY33-induced stress tolerance. Arabidopsis AtWRKY33 plays a critical role in broad plant stress responses. Whether there are evolutionarily conserved homologues of AtWRKY33 in other plants and what make AtWRKY33 such an important protein in plant stress responses are largely unknown. We compared AtWRKY33 with its close homologues to identify AtWRKY33-specific regulatory and structural elements, which were then functionally analysed through complementation. We also performed phylogenetic analysis to identify structural AtWRKY33 homologues in other plants and functionally analysed two tomato homologues through complementation and gene silencing. AtWRKY33 has an extended C-terminal domain (CTD) absent in its close homologue AtWRKY25. Both its CTD and the strong pathogen/stress-responsive expression of AtWRKY33 are necessary to complement the critical phenotypes of atwrky33. Structural AtWRKY33 homologues were identified in both dicot and monocot plants including two (SlWRKY33A and SlWRKY33B) in tomato. Molecular complementation and gene silencing confirmed that the two tomato WRKY genes play a critical role similar to that of AtWRKY33 in plant stress responses. Thus, WRKY33 proteins are evolutionarily conserved with a critical role in broad plant stress responses. Both its CTD and promoter are critical for the uniquely important roles of WRKY33 in plant stress responses.