Differential permeability of the blood-brain barrier in experimental brain metastases produced by human neoplasms implanted into nude mice.
Differential permeability of the blood-brain barrier in experimental brain metastases produced by human neoplasms implanted into nude mice.
复制标题
DOI：
--
复制
发表时间：
1992-11
期刊：
影响因子：
--
通讯作者：
Ruo-dan Zhang;J. Price;T. Fujimaki;C. Bucana;I. Fidler
中科院分区：
文献类型：
--
作者：
Ruo-dan Zhang;J. Price;T. Fujimaki;C. Bucana;I. Fidler
本研究利用水溶性荧光素钠（mw376）作为血脑屏障功能指标，阐明实验性脑转移瘤的血脑屏障是否受损以及何时受损。将8种人肿瘤细胞系（4种黑色素瘤、2种乳腺癌、1种结肠癌和1种肾癌）的细胞接种于裸鼠颈内动脉。对不同发展阶段的脑转移瘤进行采样，并测定转移瘤周围血脑屏障的通透性。组织学检查显示两种肿瘤生长模式。在第一组中，肿瘤细胞在脑实质中形成孤立的、界限明确的结节。在小于0.2 mm2的病变中，血脑屏障完好无损。在第二组，肿瘤细胞的小弥漫性巢分布在整个脑实质。血脑屏障是完整的，直到小肿瘤细胞聚集形成大肿块。这些结果表明血脑屏障的通透性在不同的脑转移瘤实验中有所不同，其功能与病变的生长方式和大小有关。
This study clarified whether and when the blood-brain barrier in experimental brain metastases is impaired by using hydrosoluble sodium fluorescein (MW 376) as a blood-brain barrier function indicator. Cells from eight human tumor lines (four melanomas, two breast carcinomas, one colon carcinoma, and one renal carcinoma) were inoculated into the internal carotid artery of nude mice. Brain metastases at different stages of development were sampled and the permeability of the blood-brain barrier around the metastases determined. Histologic examination showed two patterns of tumor growth. In the first, tumor cells formed isolated, well-defined nodules in the parenchyma of the brain. In lesions smaller than 0.2 mm2, the blood-brain barrier was intact. In the second, small diffuse nests of tumor cells were distributed throughout the brain parenchyma. The blood-brain barrier was intact until the small tumor cell colonies coalesced to form large tumor masses. These results suggest that the permeability of the blood-brain barrier varies among different experimental brain metastases and that its function is related to the growth pattern and size of the lesions.