Combination of Ginsenoside Rg1 and Bone Marrow Mesenchymal Stem Cell Transplantation in the Treatment of Cerebral Ischemia Reperfusion Injury in Rats
Combination of Ginsenoside Rg1 and Bone Marrow Mesenchymal Stem Cell Transplantation in the Treatment of Cerebral Ischemia Reperfusion Injury in Rats
复制标题
DOI：
10.1159/000430217
复制
发表时间：
2015-09-01
影响因子：
--
通讯作者：
Liu, Xia
中科院分区：
文献类型：
--
作者：
Bao, Cuifen;Wang, Yan;Liu, Xia
背景/目标：本研究旨在探讨重组人参皂苷Rg 1联合骨髓间充质干细胞（BMSC）移植对大鼠脑缺血再灌注损伤（CIRI）的保护作用及其机制。研究方法：120只雄性SD大鼠随机分为假手术组、缺血再灌注组（IR组）、BMSC移植组（IR+ BMSC）、Rg 1组（IR+ Rg 1组）、BMSC移植+Rg 1组（IR+ Rg 1 + BMSC）。采用右侧大脑中动脉栓塞法建立CIRI模型。通过神经功能评分、2，3，5-三苯基四氮唑氯化物（TTC）染色及脑含水量的测定，评价治疗效果。HE染色及TUNEL法观察病理变化及细胞凋亡情况。采用免疫荧光法检测神经元特异性烯醇化酶（NSE）和胶质细胞酸性蛋白（GFAP）的表达。Western blotting检测凋亡相关蛋白Bcl-2、Bax。结果如下：与假手术组相比，缺血再灌注组表现出明显的缺血性改变，如明显的神经功能缺损和脑含水量增加。Rg 1处理导致细胞凋亡明显减少，改善缺血状况。骨髓间充质干细胞移植后，移植细胞可分化为神经元和胶质细胞，改善脑缺血。更重要的是，IR+ Rg 1 +BMSCs组表现出最好的治疗效率，细胞凋亡减少，脑恢复更好。结论：Rg 1处理后细胞凋亡明显减少，移植细胞可分化为神经元和胶质细胞，改善脑缺血。版权所有（C）2015 S. Karger AG，巴塞尔
Background/Aims: The present study aims to explore the protective role and mechanism of ginsenoside Rg1 combined with bone marrow mesenchymal stem cell (BMSC) transplantation for cerebral ischemia reperfusion injury (CIRI) in rat brain. Methods: One hundred twenty male SD rats were randomly divided into a sham group, an Ischemia Reperfusion (IR) group, an IR group treated with BMSC transplantation(IR+BMSCs), an IR group treated with Rg1 (IR+Rg1), and an IR group treated with BMSC transplantation and Rg1 (IR+Rg1+BMSCs). To establish a CIRI model, right middle cerebral artery embolization was used. The neurological score, 2,3,5-triphenyltet-razolium chloride monohydrate (TTC) staining and brain water content were detected to assess the treatment efficiency. HE staining and TUNEL were used to explore the pathologic changes and apoptosis. To explore the protein levels of neuronspecific enolase (NSE) and glial fibrillary acidic protein (GFAP), immunofluoresence was utilized. Western blotting was used to explore apoptosis-related proteins such as Bcl-2 and Bax. Results: Compared with the sham group, the IR group demonstrated obvious ischemic changes, such as significant neurologic defects and enhanced brain water content. The Rg1 treatment resulted in an obvious decrease in cell apoptosis and improved ischemic conditions. By BMSC transplantation, the transplanted cells could be differentiated into neurons and glial cells, which also improved cerebral ischemia. More importantly, the IR+Rg1+BMSCs group showed the best treatment efficiency with reduced cell apoptosis and better cerebral recovery. Conclusions: The Rg1 treatment resulted in an obvious decrease in cell apoptosis, while the transplanted cells could be differentiated into neurons and glial cells, which also improved cerebral ischemia. Copyright (C) 2015 S. Karger AG, Basel