Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-causing Plasmodium parasites based on enzyme activity measurement.
Droplet microfluidics platform for highly sensitive and quantitative detection of malaria-causing Plasmodium parasites based on enzyme activity measurement.
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DOI：
10.1021/nn3038594
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发表时间：
2012-12-21
期刊：
影响因子：
17.1
通讯作者：
Knudsen, Birgitta R.
中科院分区：
文献类型：
--
作者：
Juul, Sissel;Nielsen, Christine J. F.;Labouriau, Rodrigo;Roy, Amit;Tesauro, Cinzia;Jensen, Pia W.;Harmsen, Charlotte;Kristoffersen, Emil L.;Chiu, Ya-Ling;Frohlich, Rikke;Fiorani, Paola;Cox-Singh, Janet;Tordrup, David;Koch, Jorn;Bienvenu, Anne-Lise;Desideri, Alessandro;Picot, Stephane;Petersen, Eskild;Leong, Kam W.;Ho, Yi-Ping;Stougaard, Magnus;Knudsen, Birgitta R.
关键词：
我们提出了一种有吸引力的新系统，用于特异性和敏感性检测引起疟疾的疟原虫。该系统依赖于由疟原虫拓扑异构酶I特异性催化的单DNA切割-连接事件的等温转化，以在单分子水平上检测到微米大小的产物。结合微滴微流体芯片实验室平台，该设计可在单滴未经处理的血液中对所有引起人类疟疾的疟原虫进行敏感、特异性和定量检测，检测限小于1个/μL。此外，该装置允许在感染患者的非侵入性唾液样本中检测疟原虫。近年来，疟疾传播在世界范围内有所下降，因此寄生虫密度低的患者人数有所增加。因此，准确发现甚至少数寄生虫的需要对继续防治该疾病变得越来越重要。我们认为，所提出的液滴微流体平台具有很高的适应潜力，适合低资源环境的护理点设置，可能有助于满足这一需求。此外，对所提出的检测其他微生物的装置进行潜在的未来调整，可能为开发更通用的诊断平台、淡水或食品质量控制平台或应用或基础科学领域的其他目的奠定基础。
We present an attractive new system for the specific and sensitive detection of the malaria causing Plasmodium parasites. The system relies on isothermal conversion of single DNA cleavage-ligation events catalyzed specifically by the Plasmodium enzyme topoisomerase I to micrometer sized products detectable at the single-molecule level. Combined with a droplet-microfluidics Lab-on-a-Chip platform, this design allowed for sensitive, specific and quantitative detection of all human malaria causing Plasmodium species in single drops of unprocessed blood with a detection limit of less than one parasite/μL. Moreover, the setup allowed for detection of Plasmodium parasites in non-invasive saliva samples from infected patients. During recent years malaria transmission has declined worldwide and with this the number of patients with low-parasite density has increased. Consequently, the need for accurate detection of even a few parasites is becoming increasingly important for the continued combat against the disease. We believe that the presented droplet-microfluidics platform, which has a high potential for adaptation to point-of-care setups suitable for low-resource settings may contribute significantly to meet this demand. Moreover, potential future adaptation of the presented setup for the detection of other microorganisms may form the basis for the development of a more generic platform for diagnosis, fresh water- or food quality control or other purposes within applied or basic science.
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影响因子：
13.3
作者：
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影响因子：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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