Overproduction of discoidin I by a temperature-sensitive motility mutant of Dictyostelium discoideum.
Overproduction of discoidin I by a temperature-sensitive motility mutant of Dictyostelium discoideum.
复制标题
盘基网柄菌温度敏感运动突变体过量生产盘基蛋白 I。
DOI：
10.1128/mcb.4.6.1035-1041.1984
复制
发表时间：
1984
影响因子：
5.3
通讯作者：
Clarke,M
中科院分区：
文献类型：
--
作者：
Biswas,S;Kayman,SC;Clarke,M
Dictyostelium disideummc2是AX3的一个温度敏感的运动突变体。突变细胞在限制性条件下不能生长、吞噬和迁移（Kayman et al.， J. Cell biol .92:705 - 711,1982）。我们在这里表明，在限制性温度下，MC2细胞不生长或在细菌上生长合成了过量的凝集素盘状蛋白I。通过二维凝胶电泳和肽图谱，MC2细胞过量产生的蛋白质与AX3细胞中较低水平合成的盘状蛋白I没有区别。三种盘孢素I中至少有两种过量生产。在27°C无菌培养基中孵育过夜的细胞中，该蛋白家族占总蛋白的9%。尽管MC2细胞在限制性条件下营养摄取有缺陷，但盘状蛋白I的过量产生似乎并不是对饥饿的多向性反应的一部分。我们认为，在突变细胞中，协调调节的盘状蛋白I基因的转录发生了改变。这种改变可能与MC2表现出的运动性缺陷有关。
Dictyostelium discoideumMC2 is a temperature-sensitive motility mutant of AX3. Mutant cells are incapable of growth, phagocytosis, and migration under restrictive conditions (Kayman et al., J. Cell Biol.92:705–711, 1982). We show here that at the restrictive temperature MC2 cells grown axenically or on bacteria synthesized excessive quantities of the lectin discoidin I. By two-dimensional gel electrophoresis and peptide mapping, the proteins overproduced by MC2 cells were indistinguishable from discoidin I synthesized at lower levels in AX3 cells. At least two of the three species of discoidin I were overproduced. This protein family constituted 9% of the total protein in cells that were incubated overnight at 27°C in axenic medium. Although MC2 cells are defective in nutrient uptake under restrictive conditions, the overproduction of discoidin I did not appear to be part of a pleiotropic response to starvation. We propose that transcription of the coordinately regulated discoidin I genes is altered in mutant cells. This alteration may be related to the motility defects manifested by MC2.