Enhanced light collection in a flow cytometer.
Enhanced light collection in a flow cytometer.
复制标题
增强流式细胞仪中的光收集。
DOI：
10.1002/cyto.990010106
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Coulter,JR
中科院分区：
文献类型：
--
作者：
Fox,MH;Coulter,JR
描述了常规流式细胞仪中的改进的流动池。分析了光在流动池-空气界面中的折射。分析表明，可以收集的大部分光被折射出收集光学器件。通过向流动池添加平凸透镜，光收集效率加倍。此外，通过添加第二鞘实现了流动池的更稳定的操作。
A modified flow cell in a conventional flow cytometer is described. The refraction of light in the flow cell‐to‐air interface is analyzed. The analysis shows that much of the light that can be collected is refracted out of the collection optics. The efficiency of light collection is doubled by the addition of a plano‐convex lens to the flow cell. Also, more stable operation of the flow cell is achieved with the addition of a secondary sheath.