THE LARGE-SCALE ISOLATION OF BRYOSTATIN-1 FROM BUGULA-NERITINA FOLLOWING CURRENT GOOD MANUFACTURING PRACTICES
THE LARGE-SCALE ISOLATION OF BRYOSTATIN-1 FROM BUGULA-NERITINA FOLLOWING CURRENT GOOD MANUFACTURING PRACTICES
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DOI：
10.1021/np50077a004
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发表时间：
1991-09-01
影响因子：
5.1
通讯作者：
FORENZA, S
中科院分区：
文献类型：
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作者：
SCHAUFELBERGER, DE;KOLECK, MP;FORENZA, S
设计了一种从草苔虫中大规模分离苔藓抑素1的新工艺。以获得数克量的高纯材料用于癌症患者的制剂研究、临床前毒理学和临床试验。 从收集的约10，000加仑的湿动物中获得数克量的苔藓抑素1。 使用二丁酸佛波酯（PDBu）受体结合试验和具有光电二极管阵列检测的hplc进行分离工艺的设计、验证和控制。
A novel process was designed for the large-scale isolation of bryostatin 1 {1} from the bryozoan Bugula neritina L. in order to obtain multigram quantities of highly pure material for formulation studies, preclinical toxicology, and clinical trials in cancer patients. Multigram quantities of bryostatin 1 were obtained from a collection of approximately 10,000 gallons of wet animal. A phorbol dibutyrate (PDBu) receptor binding assay and hplc with photodiode array detection were used for the design, validation, and control of the isolation process.