EFFECTS OF HUANG JING ZAN YU CAPSULE ON OLIGOASTHENOZOOSPERMIA RATS SPERM MOTILITY AND GENETIC EXPRESSION PROFILES
EFFECTS OF HUANG JING ZAN YU CAPSULE ON OLIGOASTHENOZOOSPERMIA RATS SPERM MOTILITY AND GENETIC EXPRESSION PROFILES
复制标题
黄精瓒郁胶囊对少精症大鼠精子活力及基因表达谱的影响
作者：
BOYI LI;CHENGLONG ZHENG;KAILIN ZHOU;ZHAOZHU JIAO;JI YAN;QI WANG;YANFEI ZHENG
本研究旨在通过微阵列技术观察黄精蠲郁胶囊对少弱精子症大鼠基因表达谱及精子的影响。用环磷酰胺诱导大鼠睾丸损伤形成少弱精子症模型，左卡尼汀为对照组。治疗组给予黄经散郁胶囊，疗程2周。然后利用精子质量检测系统对精子密度、活力等指标进行检测。利用全基因组Oligo芯片分析基因表达谱的变化。黄精蠲饮胶囊a、b组小鼠精子数量、密度、活力、活力均有不同程度改善，与对照组比较差异有统计学意义（P < 0.05）。差异表达基因分散在8条重要的信号通路上，分别是细胞凋亡通路、细胞粘附分子信号通路、神经营养因子信号通路、嗅觉转导通路、类固醇激素生物合成通路、代谢通路、视黄醇代谢通路和花生四烯酸代谢通路。6个新的关键基因Adam7、Lcn5、Slc7a8、Hoxa10、Slc4a9、Vom2r57被确认。黄精瓒育胶囊可提高少弱精子症大鼠精子活力。其机制与凋亡信号通路、细胞粘附分子信号通路、神经营养因子信号通路、嗅觉信号通路、类固醇激素生物合成途径、代谢途径、视黄醇代谢途径和花生四烯酸代谢途径等八大信号通路有关。
The aim of the paper was to observe the effect of Huang Jing Zan Yu capsule on oligoasthenozoospermia rats genetic expression profiles and sperm through microarray method. Cyclophosphamide was used to induce injury of rats testis as to form oligoasthenozoospermia model, while levocarnitine in control group. Huang Jing Zan Yu capsule was administered to treatment group for 2 weeks. Then detection system of sperm quality was used to determine indexes such as sperm density and motility. Genome-wide Oligo chips were used to analyse the changes of genetic expression profiles. The sperm amount, density, motility, and viability of Huang Jing Zan Yu capsule a level and b level had different degree improvements, and had clear differences compared with the control group (P < 0.05). Differential expression genes were scattered in eight prominent signalling pathways, which were apoptosis pathways, cellular adhesion molecule signalling pathways, neurotrophic factor signalling pathways,olfaction transduction pathways, steroid hormone biosynthesis pathways, metabolic pathways, retinol metabolism pathways and arachidonic acid metabolism pathways. Six new key genes, Adam7, Lcn5, Slc7a8, Hoxa10, Slc4a9, Vom2r57 were confirmed.Huang Jing Zan Yu capsule can improve sperm vitality of oligoasthenozoospermia rats. The mechanism is associated with eight prominent signalling pathways, which are apoptosis pathways, cellular adhesion molecule signalling pathways, neurotrophic factor signalling pathways, olfaction transduction pathways, steroid hormone biosynthesis pathways, metabolic pathways, retinol metabolism pathways and arachidonic acid metabolism pathways.