Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast.
Identification of novel secreted fatty acids that regulate nitrogen catabolite repression in fission yeast.
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DOI：
10.1038/srep20856
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发表时间：
2016-02-19
影响因子：
4.6
通讯作者：
Yashiroda Y
中科院分区：
文献类型：
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作者：
Sun X;Hirai G;Ueki M;Hirota H;Wang Q;Hongo Y;Nakamura T;Hitora Y;Takahashi H;Sodeoka M;Osada H;Hamamoto M;Yoshida M;Yashiroda Y
在优质氮源如NH 4+和谷氨酸（Glu）存在下，对贫氮源如支链氨基酸的摄取受到抑制，这称为氮分解代谢物抑制。氨基酸营养缺陷型突变体的裂殖酵母裂殖酵母不能生长在基本培养基上含有氯化铵或谷氨酸，即使当足够的量所需的氨基酸供应。然而，这些突变体细胞的生长在原养型菌株的菌落附近恢复，表明原养型细胞分泌一些物质，可以通过未知的机制恢复氨基酸的摄取。我们鉴定了新的脂肪酸，10（R）-乙酰氧基-8（Z）-十八碳烯酸和10（R）-羟基-8（Z）-十八碳烯酸，作为分泌的活性物质，称为氮信号因子（NSFs）。合成NSF还能够将氮源利用从优质氮源转移到劣质氮源，以允许裂变酵母氨基酸营养缺陷型突变体在优质氮源存在下的适应性生长。最后，我们证明了Agp 3氨基酸转运蛋白参与了适应性生长。这些数据突出了一种新的种内通信系统，用于适应裂变酵母的环境营养条件。
Uptake of poor nitrogen sources such as branched-chain amino acids is repressed in the presence of high-quality nitrogen sources such as NH4+ and glutamate (Glu), which is called nitrogen catabolite repression. Amino acid auxotrophic mutants of the fission yeast Schizosaccharomyces pombe were unable to grow on minimal medium containing NH4Cl or Glu even when adequate amounts of required amino acids were supplied. However, growth of these mutant cells was recovered in the vicinity of colonies of the prototrophic strain, suggesting that the prototrophic cells secrete some substances that can restore uptake of amino acids by an unknown mechanism. We identified the novel fatty acids, 10(R)-acetoxy-8(Z)-octadecenoic acid and 10(R)-hydroxy-8(Z)-octadecenoic acid, as secreted active substances, referred to as Nitrogen Signaling Factors (NSFs). Synthetic NSFs were also able to shift nitrogen source utilization from high-quality to poor nitrogen sources to allow adaptive growth of the fission yeast amino acid auxotrophic mutants in the presence of high-quality nitrogen sources. Finally, we demonstrated that the Agp3 amino acid transporter was involved in the adaptive growth. The data highlight a novel intra-species communication system for adaptation to environmental nutritional conditions in fission yeast.