Albumin adsorption and retention on C18-alkyl-derivatized polyurethane vascular grafts.
Albumin adsorption and retention on C18-alkyl-derivatized polyurethane vascular grafts.
复制标题
白蛋白在 C18-烷基衍生的聚氨酯血管移植物上的吸附和保留。
DOI：
10.1111/j.1525-1594.1987.tb00948.x
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发表时间：
1987
影响因子：
2.4
通讯作者：
Fry,WJ
中科院分区：
文献类型：
--
作者：
Eberhart,RC;Munro,MS;Williams,GB;Kulkarni,PV;ShannonJr,WA;Brink,BE;Fry,WJ
聚醚聚氨酯血管移植物的短期白蛋白亲和力和抗血栓性得到了改善。该方法基于聚合物的C18烷基化。在犬股动脉部位短期（4 h）暴露时，通过平面测量技术测量血栓形成和钢丝增强聚氨酯管（C18烷基化和未处理）上的白蛋白保留。将125 I-白蛋白预吸附在管上，然后连续暴露于血液2 h。血液暴露前烷基化试管的白蛋白摄取量和血液暴露2小时后的保留量显著高于对照组。在血液中快速解吸阶段后，剩余的白蛋白从烷基化试管中解吸的速度比从对照试管中更慢。与白蛋白再孵育和血液再暴露产生了类似的趋势，表明血液调节在短期内不会降低C18烷基化的白蛋白亲和力增强特性。血液预处理实验表明内源性白蛋白对C18烷基化表面具有高亲和力。扫描电子显微镜检查显示，血栓和血小板密度高于控制比烷基化表面。这些结果表明，C18烷基化聚氨酯的体内白蛋白亲和力增加，这可能与这些表面上血栓形成减少有关。
The short‐term albumin affinity and thrombo‐resistance of a polyether polyurethane vascular graft have been improved. The method is based on the C18alkylation of the polymer. Thrombus formation by a planimetric technique and albumin retention on wire‐reinforced polyurethane tubes, both C18alkylated and untreated, were measured in short‐term (4‐h) exposure at femoral arterial sites in the dog.125I‐Albumin was preadsorbed on tubes and then exposed to blood for successive 2‐h periods. Albumin uptake on alkylated tubes prior to blood exposure and retention following 2 h of blood exposure were significantly greater than on controls. Following a fast desorption phase in blood, the remaining albumin was more slowly desorbed from alkylated than from control tubes. Reincubation with albumin and blood reexposure produced a similar tendency, suggesting blood conditioning does not reduce the albumin affinity‐enhancing property of C18alkylation in the short term. Blood‐preconditioning experiments suggested endogenous albumin has a high affinity for the C18‐alkylated surface. Scanning electron microscopic examination showed thrombus and platelet densities were higher on control than on alkylated surfaces. These results suggest in vivo albumin affinity is increased for C18‐alkylated polyurethane, which may be linked to decreased thrombus formation on these surfaces.