Prognostic value of EphA2 and EphrinA-1 in squamous cell cervical carcinoma
Prognostic value of EphA2 and EphrinA-1 in squamous cell cervical carcinoma
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DOI：
10.1016/j.ygyno.2004.05.019
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发表时间：
2004-08-01
影响因子：
4.7
通讯作者：
Nesland, JM
中科院分区：
文献类型：
--
作者：
Wu, D;Suo, ZH;Nesland, JM
目标。分析EphA2和EphrinA-1蛋白及mRNA在宫颈鳞状细胞癌中的表达，探讨其对宫颈癌预后的价值。采用免疫组化方法检测206例FIGO Ia~IVb期宫颈鳞癌患者EphA2和EphrinA-1蛋白表达情况。来自 20 例肿瘤显示不同 EphA2 和 EphrinA-1 蛋白表达的冷冻组织用于激光捕获显微切割 (LCM)。用LCM方法捕获每个冰冻切片中约50个癌细胞，并进行RT-PCR检测EpbA2和EpbrinA-1 mRNA。结果。 206例宫颈鳞癌中，EphA2免疫染色阴性23例（11.2%），弱阳性94例（45.6%），中度阳性72例（35.0%），强阳性17例（8.3%）。对于EphrinA-1蛋白表达，17个肿瘤（8.3%）呈阴性，95个（46.1%）弱阳性，71个（34.5%）中度阳性，23个（11.2%）强阳性。 EphA2 和 EphrinA-1 通常共定位于相同的肿瘤区域和血管内皮细胞。在分析的 20 个肿瘤中观察到 EpbA2 和 EphrinA-1 mRNA 表达量的变化。 EphA2/EphrinA-1过表达与年龄、FIGO分期无显着相关性。在单变量和多变量分析中，高水平的 EphA2 与总生存率显着相关。多变量分析显示，中等到高 EphrinA-1 蛋白表达与总生存率显着相关。 EhpA2 的高水平表达和 EphrinA-1 的中至高水平表达相结合是总生存率的有力预测因子。结论。鳞状宫颈癌中高水平的 EphA2 以及中等到高水平的 EphrinA-1 蛋白表达预示着较短的总生存期，这些蛋白可能是有价值的预后标志物。 (C) 2004 Elsevier Inc. 保留所有权利。
Objectives. To analyze the expressions of the protein and mRNA of EphA2 and EphrinA-1 in squamous cell cervical carcinomas and explore their prognostic value in cervical carcinoma.Methods. Immunohistochemistry was used to assess the protein expressions of EphA2 and EphrinA-1 in 206 patients with squamous cervical carcinoma FIGO stage Ia-IVb. Frozen tissues from 20 cases in which the tumors showed variable EphA2 and EphrinA-1 protein expressions were used for laser capture microdissection (LCM). About 50 cancer cells in each frozen section were captured with the LCM method and processed for RT-PCR detection of EpbA2 and EpbrinA-1 mRNA.Results. Among the 206 squamous cervical carcinomas, 23 (11.2%) showed negative, 94(45.6%) weakly positive, 72 (35.0%) moderately positive, and 17 (8.3%) strongly positive for EphA2 immunostaining. For EphrinA-1 protein expression, 17 tumors (8.3%) showed negative, 95 (46.1%) weakly positive, 71(34.5%) moderately positive, and 23 (11.2%) strongly positive. EphA2 and EphrinA-1 often colocalized in the same tumor areas and vascular endothelial cells. Variable amount of mRNA expressions of EpbA2 and EphrinA-1 were observed in the 20 tumors analyzed. There was no significant correlation between the overexpressions of EphA2/EphrinA-1 and age and FIGO stage. High level of EphA2 was significantly associated with overall survival in univariate and multivariate analysis. Moderate to high EphrinA-1 protein expression was significantly associated with overall survival in multivariate analysis. The combined high level of expression of EhpA2 and moderate to high level of expression of EphrinA-1 was a strong predictor of overall survival.Conclusions. High levels of EphA2 together with moderate to high level of EphrinA-1 protein expressions in squamous cervical carcinoma were predictive for a shorter overall survival and these proteins may be valuable prognostic markers. (C) 2004 Elsevier Inc. All rights reserved.