Reproductive barriers to hybridizations between narrow-leaf and broad-leaf Anoectochilus roxburghii
Reproductive barriers to hybridizations between narrow-leaf and broad-leaf Anoectochilus roxburghii
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DOI：
10.1080/14620316.2016.1240594
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发表时间：
2017-03
期刊：
影响因子：
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通讯作者：
Renda Teng;Qingsong Shao;Mei Wu;Hongzheng Wang;Ming-yan Li;Yuqiu Huang
中科院分区：
文献类型：
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作者：
Renda Teng;Qingsong Shao;Mei Wu;Hongzheng Wang;Ming-yan Li;Yuqiu Huang
摘要以窄叶金线莲和宽叶金线莲为正反交亲本，探讨其结实率低的原因。用荧光显微镜观察花粉-雌蕊相互作用，用石蜡切片技术观察杂种胚的发育。结果表明，花粉管生长可达胚囊，完成双受精。这些结果表明，受精前障碍不是影响窄叶和阔叶A杂交的主要因素。刺梨。杂种胚乳发育异常，最终导致胚败育。受精后障碍可能是窄叶型与宽叶型杂交结实率低的主要原因。刺梨。此外，一个有效的胚胎拯救协议，其中的发展在其他地方报道，被用来比较之间的相互杂交拯救所需的时间。
ABSTRACT Narrow-leaf and broad-leaf Anoectochilus roxburghii were used as reciprocal parents to explore the reason for low seed setting rate. Pollen-pistil interaction was observed by fluorescence microscopy and hybrid embryo development by paraffin section technology. The results indicated that pollen tube growth could reach the embryo sac, and double fertilization could be completed. These findings showed that a pre-fertilization barrier was not the major factor in the cross of narrow-leaf and broad-leaf A. roxburghii. The endosperm development of the hybrid was abnormal and eventually promoted abortion of the embryos. A post-fertilization barrier appears to be the major factor for low seed setting rate in the cross of narrow-leaf and broad-leaf A. roxburghii. In addition, an efficient protocol of embryo rescue, the development of which is reported elsewhere, was used to compare the time needed for rescue between the reciprocal crosses.