Effect of DHA-rich fish oil on PPARγ target genes related to lipid metabolism in type 2 diabetes: A randomized, double-blind, placebo-controlled clinical trial
Effect of DHA-rich fish oil on PPARγ target genes related to lipid metabolism in type 2 diabetes: A randomized, double-blind, placebo-controlled clinical trial
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DOI：
10.1016/j.jacl.2015.08.007
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发表时间：
2015-12-01
影响因子：
4.4
通讯作者：
Koohdani, Fariba
中科院分区：
文献类型：
--
作者：
Mansoori, Anahita;Sotoudeh, Gity;Koohdani, Fariba
背景：omega-3多不饱和脂肪酸对血脂水平的有益影响是有文献记载的。然而，相关的分子机制却鲜为人知。Omega-3多不饱和脂肪酸是过氧化物酶体增殖物激活受体γ(PPAR Gamma)的天然配体。目的：本研究的目的是评价富含二十二碳六烯酸(DHA)的鱼油对一些与脂代谢相关的PPAR伽马反应基因的调节作用。方法：2型糖尿病患者随机分为两组，分别摄入富含DHA的鱼油(含2400 mg/d鱼油；DHA：1450 mg和二十碳五烯酸：400 mg)和安慰剂8周。结果：与安慰剂组相比，补充富含DHA鱼油可降低甘油三酯(TG)水平，且与基础值无关(所有患者(P=.003)、高甘油三酯血症患者(P=.01)和正常甘油三酯血症患者(P=.02))。此外，与服用富含DHA鱼油的正常甘油三酯血症受试者相比，高甘油三酯血症受试者的甘油三酯水平下降幅度更大(P=0.01)。其他脂质参数以及PPAR-γ、肝脏x受体-a、ATP结合盒A1和CD36的表达不受富含DHA的鱼油的影响。仅在高甘油三酯血症患者中，与安慰剂组相比，富含DHA鱼油可上调CD36的表达(P=.01)。结论：与正常甘油三酯血症患者相比，补充富含DHA鱼油8周可增加高甘油三酯血症患者CD36的表达，这可能导致甘油三酯水平下降幅度更大。然而，这一发现应该在进一步的研究中进行调查。(C)2015年全国油脂协会。版权所有。
BACKGROUND: The beneficial effects of omega-3 polyunsaturated fatty acids on lipid levels are well documented. However, the related molecular mechanisms are widely unknown. Omega-3 polyunsaturated fatty acids are natural ligand for peroxisome proliferator activated receptor gamma (PPAR gamma).OBJECTIVE: The aim of this study was to evaluate the effect of docosahexaenoic acid (DHA)-rich fish oil supplementation on modulation of some PPAR gamma-responsive genes related to lipid metabolism.METHODS: Patients with type 2 diabetes were randomly assigned to consume either DHA-rich fish oil (containing 2400 mg/d fish oil; DHA: 1450 mg and eicosapentaenoic acid: 400 mg) or placebo for 8 weeks. Lipid profile and glycemic control parameters as well as the gene expression of PPAR gamma, liver x receptor-a, ATP-binding cassette A1, and CD36 in peripheral blood mononuclear cells were measured at baseline and after 8 weeks.RESULTS: DHA-rich fish oil supplementation resulted in decreased triglycerides (TG) level compared with placebo group, independently of the baseline value of TG (all patients (P = .003), hypertriglyceridemic subjects (P = .01), and normotriglyceridemic subjects (P = .02)). Moreover, a higher reduction in TG level was observed in hypertriglyceridemic subjects, comparing to normotriglyceridemic subjects with DHA-rich fish oil supplementation (P = .01). Other lipid parameters as well as the expression of PPAR gamma, liver x receptor-a, ATP-binding cassette A1, and CD36 were not affected by DHA-rich fish oil supplementation. Only in hypertriglyceridemic subjects, DHA-rich fish oil supplementation upregulated CD36 expression, compared with the placebo group (P = .01).CONCLUSIONS: DHA-rich fish oil supplementation for 8 weeks increased CD36 expression in hypertriglyceridemic subjects, which might result to higher reduction in TG level, comparing with normotriglyceridemic subjects. However, this finding should be investigated in further studies. (C) 2015 National Lipid Association. All rights reserved.