Interrelation between long-chain fatty acid oxidation rate and carnitine palmitoyltransferase 1 activity with different isoforms in rat tissues
Interrelation between long-chain fatty acid oxidation rate and carnitine palmitoyltransferase 1 activity with different isoforms in rat tissues
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DOI：
10.1016/j.lfs.2004.11.032
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发表时间：
2005-06-10
期刊：
影响因子：
6.1
通讯作者：
Kim, JY
中科院分区：
文献类型：
--
作者：
Doh, KO;Kim, YW;Kim, JY
本研究检查了含有 L-CPT1 或 M-CPT1 的各种组织中长链脂肪酸 (LCFA) 氧化速率与肉碱棕榈酰转移酶 (CPT) 1 活性之间的相互关系。检查从斯普拉格-道利大鼠获得的肝脏、肾脏、心脏、白色和红色腓肠肌以及白色和棕色脂肪组织。在含有 L-CPT1 的组织中，肝脏的棕榈酸酯氧化率和 CPT 1 活性显着高于肾脏（P < 0.01）。在含有M-CPT1的组织中，棕色脂肪组织表现出最高的棕榈酸酯氧化率和CPT1活性。含有M-CPTI的组织（r(2)=0.907，p < 0.001）显示棕榈酸酯氧化速率与CPT1活性之间存在很强的正相关性。计算棕榈酸酯氧化速率与CPT1活性的比率。各组织中肝脏的比例最高，肾脏的比例最低。含有M-CPT1的组织的比例相似。这些结果表明，含有M-CPT1的组织中的LCFA氧化速率与CPTI活性成正比，但与含有L-CPT1的组织中的CPT1活性不成正比。总之，CPT1活性似乎对LCFA氧化非常重要，但它可能不是LCFA氧化的唯一限速步骤。 (c) 2005 Elsevier Inc. 保留所有权利。
This study examined the interrelation between the long-chain fatty acid (LCFA) oxidation rate and the carnitine palmitoyltransferase (CPT) 1 activity in various tissues containing L-CPT1 or M-CPT1. The Liver, kidney, heart, white and red gastrocnemius muscles, and white and brown adipose tissues obtained from Sprague-Dawley rats were examined. In the tissues containing L-CPT1 the liver showed a significantly higher (P < 0.01) palmitate oxidation rate and CPT 1 activity than the kidney. Among the tissues containing M-CPT1, the brown adipose tissue showed the highest palmitate oxidation rate and CPT1 activity. The tissues containing M-CPTI (r(2)=0.907, p < 0.001) showed a strong positive correlation between the palmitate oxidation rate and the CPT1 activity. The ratios of the palmitate oxidation rate to the CPT1 activity were calculated. The ratio in the liver was highest and the ratio in the kidney was lowest among the tissues. The ratios of the tissues containing M-CPT1 were similar. These results showed that the LCFA oxidation rates in the tissues containing M-CPT1 were directly proportional to the CPTI activity, but not similarly proportional to the CPT1 activity in the tissues containing L-CPT1 In conclusion, CPT1 activity seems very important factor for LCFA oxidation, but it might be not the only rate-limiting step in LCFA oxidation. (c) 2005 Elsevier Inc. All rights reserved.