Preoperative Granulocyte-Colony Stimulating Factor (G-CSF) Treatment Improves Congested Liver Regeneration
Preoperative Granulocyte-Colony Stimulating Factor (G-CSF) Treatment Improves Congested Liver Regeneration
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DOI：
10.1016/j.jss.2008.09.002
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发表时间：
2010-01-01
影响因子：
2.2
通讯作者：
Takemura, Hirofumi
中科院分区：
文献类型：
--
作者：
Kimura, Masaki;Yamada, Takuya;Takemura, Hirofumi
背景。肝手术后肝静脉充血可引起肝功能衰竭。我们评估了粒细胞集落刺激因子（G-CSF）对静脉充血残肝再生的有效性。材料与方法。大鼠被分为三组。A组行60%肝切除术。B组行60%肝切除术，残肝部分静脉充血。C组与B组相同，术前给予G-CSF 5 d。为评价各组术后1、2、3、5、7 d的肝再生情况，测量残肝充血区增殖细胞核抗原（PCNA）标记指数（LI）、残肝有丝分裂指数（MI）和残肝重量再生率。免疫病理染色法鉴定CD34抗体阳性造血干细胞（HSC）集落。与B组比较，C组充血残肝LI、MI升高较早，充血区LI峰值出现时间较早24 h，残肝重量完全恢复时间较早2 d。术后第3天，C组残肝重量明显大于b组。显微镜下，术前C组肝脏可见cd34阳性细胞。术前G-CSF可促进静脉充血的肝脏再生。(C) 2010爱思唯尔公司版权所有。
Background. Hepatic venous congestion after liver surgery can cause liver failure. We evaluated the effectiveness of granulocyte-colony stimulating factor (G-CSF) for regeneration of remnant liver with venous congestion.Materials and Methods. Rats were divided into three groups. Group A underwent 60% hepatectomy. Group B underwent 60% hepatectomy with partial venous congestion in remnant liver. Group C underwent the same procedures as group B with G-CSF given preoperatively for 5 d. To evaluate liver regeneration in each group at 1, 2, 3, 5, and 7 d postoperatively, the proliferating cell nuclear antigen (PCNA) labeling index (LI), mitotic index (MI) in the congested area of remnant liver, and regeneration rate of remnant liver weight were measured. CD34 antibody-positive hematopoietic stem cell (HSC) colonies were identified using immunopathological staining.Results. Compared with group B, in group C, LI, and MI in congested remnant liver increased earlier, peak LI value in the congested area appeared 24 h earlier, and full recovery of remnant liver weight occurred 2 d earlier. On postoperative day 3, remnant liver weight was significantly greater in group C than group B. On microscopy, CD34-positive cells were seen in preoperative group C livers.Conclusion. Preoperative G-CSF improves regeneration of livers with venous congestion. (C) 2010 Elsevier Inc. All rights reserved.