The human cathelicidin peptide LL-37 and truncated variants induce segregation of lipids and proteins in the plasma membrane of Candida albicans
The human cathelicidin peptide LL-37 and truncated variants induce segregation of lipids and proteins in the plasma membrane of Candida albicans
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DOI：
10.1515/bc.2006.187
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发表时间：
2006-10-01
影响因子：
3.7
通讯作者：
Amerongen, Arie V. Nieuw
中科院分区：
文献类型：
--
作者：
den Hertog, Alice L.;van Marle, Jan;Amerongen, Arie V. Nieuw
人凯萨林菌素肽LL-37和几种截短的变体在它们穿过白色念珠菌的质膜的能力上不同。我们研究了是否保留在细胞周边或膜迁移影响他们的膜破坏活动和杀念珠菌的性能。使用荧光素标记的肽，我们证明，LL-37和其C-末端截短肽LL-31保持永久与周边的细胞。的N-末端截短的肽RK-31最初积累在细胞边界，但transmigration进入细胞质内30分钟。的C-末端截短的肽LL-25 transmigration瞬间进入细胞质。用冷冻断裂电镜结合菲律平细胞化学研究了对质膜超微结构的影响。所有的肽，无论它们是否穿过质膜，都诱导质膜中的相分离。所有的肽都诱导细胞组分的泄漏，包括核苷酸和蛋白质。通过SDS-PAGE结合质谱鉴定蛋白质，其显示主要是小于50 kDa的蛋白质从C.白色念珠菌。
The human cathelicidin peptide LL-37 and several truncated variants differ in their capability to transmigrate over the plasma membrane of Candida albicans. We investigated whether retention at the cell perimeter or membrane transmigration affects their membrane-disrupting activities and candidacidal properties. Using fluorescein-labeled peptides, we demonstrate that LL-37 and its C-terminally truncated peptide LL-31 remain permanently associated with the perimeter of the cell. The N-terminally truncated peptide RK-31 initially accumulated at the cell boundary, but transmigrated into the cytoplasm within 30 min. The C-terminally truncated peptide LL-25 transmigrated instantaneously into the cytoplasm. The ultrastructural effects on the plasma membrane were studied by freeze-fracture electron microscopy combined with filipin cytochemistry. All peptides, whether they transmigrated over the plasma membrane or not, induced phase separation in the plasma membrane. All peptides induced leakage of cell components, including nucleotides and proteins. Proteins were identified by SDS-PAGE in combination with mass spectrometry, which revealed that predominantly proteins smaller than 50 kDa had leaked out of C. albicans.