ADENINE SPECIFIC DNA CHEMICAL SEQUENCING REACTION
ADENINE SPECIFIC DNA CHEMICAL SEQUENCING REACTION
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DOI：
10.1093/nar/15.19.7823
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发表时间：
1987-10-12
影响因子：
14.9
通讯作者：
DERVAN, PB
中科院分区：
文献类型：
--
作者：
IVERSON, BL;DERVAN, PB
DNA与K2PdCl 4在pH2.0下反应，随后进行哌啶后处理，在腺嘌呤（A）残基处产生特异性切割。产物分析显示K2PdCl 4反应涉及腺嘌呤的选择性脱嘌呤，提供类似于其他化学DNA测序反应的切除反应。在环外胺（N6）处甲基化的腺嘌呤残基的反应效率低于相同序列中未甲基化的腺嘌呤。这个简单的协议具体的A可能是一个有用的除了目前的化学测序反应。
Reaction of DNA with K2PdCI4at pH2.0 followed by a piperidine workup produces specific cleavage at adenine (A) residues. Product analysis revealed the K2PdCI4reaction involves selective depurination at adenine, affording an excision reaction analogous to the other chemical DNA sequencing reactions. Adenine residues methylated at the exocyclic amine (N6) react with lower efficiency than unmethylated adenine in an identical sequence. This simple protocol specific for A may be a useful addition to current chemical sequencing reactions.